Jean Mayer USDA- Human Nutrition Research Center on Aging, Tufts University, 711 Washington St., Boston, MA 02111, USA. ; Department of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Distrito Rubiao Jr. s/n, 18618-970 Botucatu, SP, Brazil.
Jean Mayer USDA- Human Nutrition Research Center on Aging, Tufts University, 711 Washington St., Boston, MA 02111, USA.
Nutr Res Pract. 2014 Oct;8(5):550-7. doi: 10.4162/nrp.2014.8.5.550. Epub 2014 Sep 15.
BACKGROUND/OBJECTIVES: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo.
Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.
After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05).
Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.
背景/目的：当归是一种绿叶蔬菜，富含植物色素类生物活性物质，如类黄酮和类胡萝卜素。本研究旨在探讨当归中类黄酮和类胡萝卜素的生物利用度以及体内抗氧化性能的变化。
健康老年人（>60 岁，n=5）和代谢综合征患者（n=5）分别摄入 5g 用明胶胶囊包裹的当归干粉和低类黄酮、类胡萝卜素的液体餐。在基线、0.5、1、2、3、4、5、6、7 和 8 小时采集血浆样本。采用高效液相色谱系统（电化学和紫外检测）分别分析黄酮类和类胡萝卜素，荧光法分析总抗氧化能力。
健康组中，摄入当归后 1-3 和 6-8 小时，血浆槲皮素浓度升高，与基线相比差异有统计学意义（P<0.05）；代谢综合征组所有时间点，血浆叶黄素浓度均显著升高（P<0.05）。与基线相比，健康组和代谢综合征组的总抗氧化能力均显著升高（P<0.05）。
本研究结果清楚地表明了当归中植物营养素的生物利用度及其提高人体抗氧化状态的能力。
