幽门螺杆菌vacA、cagA和iceA基因型的流行情况及其与临床结局的相关性。
Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome.
作者信息
Wei Guo-Chao, Chen Jing, Liu Ai-Yun, Zhang Miao, Liu Xiao-Jun, Liu Dan, Xu Jun, Liu Bing-Rong, Ling Hong, Wu Hua-Xing, DU Ya-Ju
机构信息
Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University; ; Department of Microbiology, Harbin Medical University;
出版信息
Exp Ther Med. 2012 Dec;4(6):1039-1044. doi: 10.3892/etm.2012.704. Epub 2012 Sep 11.
The aim of this study was to assess the genetic status of cagA, vacA subtype and iceA genotypes of Helicobacter pylori and the relationship with upper gastrointestinal diseases in Northeast China. Gastric biopsies were obtained from 378 patients with upper gastrointestinal diseases and 197 samples were used. The cagA, vacA alleles and iceA genotypes were determined by polymerase chain reaction. CagA was present in 176 (89.3%) of 197 patients. Of the 197 cases, 186 (94.4%) had vacA signal sequence s1c allele, 6 (3%) had s1a and 5 (2.5%) had s1b. The vacA s2 genotype was not detected in our study. VacA middle region sequences, m1 and m2, were found in 20 (10.2%) and 150 (76.1%), respectively. The allelic variant iceA1 (70.1%) was more prevalent than iceA2 (23.4%). The vacA allele s1am2 had a significant relationship with the presence of gastric cancer (p<0.05) and the iceA1 genotype was also associated with gastric cancer (p<0.05). These may be useful risk factors for upper gastrointestinal diseases.
本研究旨在评估中国东北地区幽门螺杆菌的cagA、vacA亚型和iceA基因型的遗传状况及其与上消化道疾病的关系。从378例上消化道疾病患者中获取胃活检组织，共使用197份样本。通过聚合酶链反应测定cagA、vacA等位基因和iceA基因型。197例患者中，176例（89.3%）检测到cagA。在这197例中，186例（94.4%）具有vacA信号序列s1c等位基因，6例（3%）具有s1a，5例（2.5%）具有s1b。本研究未检测到vacA s2基因型。分别在20例（10.2%）和150例（76.1%）中发现VacA中间区域序列m1和m2。等位基因变异体iceA1（70.1%）比iceA2（23.4%）更常见。vacA等位基因s1am2与胃癌的发生存在显著关系（p<0.05），iceA1基因型也与胃癌相关（p<0.05）。这些可能是上消化道疾病有用的危险因素。
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