韩国牛分枝杆菌的多位点序列分型和多位点可变数目串联重复分析的基因分型。

Genotyping of from Cattle by Multispacer Sequence Typing and Multiple Locus Variable Number of Tandem Repeat Analysis in the Republic of Korea.

机构信息

Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Korea.

Faculty of Biotechnology, Thai Nguyen University of Sciences, Thai Nguyen 250000, Vietnam.

出版信息

Genes (Basel). 2022 Oct 23;13(11):1927. doi: 10.3390/genes13111927.

DOI:10.3390/genes13111927

PMID:36360164

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9689931/

Abstract

Genotyping of using multispacer sequence typing (MST) and multiple locus variable number tandem repeat analysis (MLVA) was conducted from infected animals for the first time in the Republic of Korea. was detected by real-time PCR, and followed by MST and MLVA genotyping. The result showed that detected all had the same MLVA genotype, 6-13-2-7-9-10 for markers MS23-MS24-MS27-MS28-MS33-MS34, respectively, and genotype group 61 for MST. The same genotypes were previously identified in Poland. Importantly, this MLVA type was detected in humans in France, suggesting that the Korean strain can also potentially cause Q fever in humans. MST and MLVA were very useful tools for analyzing the molecular epidemiology of and helpful for interpreting the epidemiological relationship between isolates from domestic and international resources.

摘要

首次在韩国对感染动物进行了应用多位点间隔重复序列分型（MST）和多位点可变数目串联重复分析（MLVA）的基因分型。通过实时 PCR 检测到，随后进行 MST 和 MLVA 基因分型。结果表明，检测到的所有均具有相同的 MLVA 基因型，分别为标记 MS23-MS24-MS27-MS28-MS33-MS34 的 6-13-2-7-9-10，以及 MST 的基因型组 61。在波兰也发现了相同的基因型。重要的是，这种 MLVA 类型在法国的人类中被检测到，这表明韩国株也有可能在人类中引起 Q 热。MST 和 MLVA 是分析分子流行病学的非常有用的工具，并有助于解释来自国内外资源的分离株之间的流行病学关系。