State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2013 Oct;145:259-63. doi: 10.1016/j.biortech.2012.11.020. Epub 2012 Nov 21.
This study isolated a total of seven pure cultures from activated sludge that could produce bioflocculants from 1.7% v/v H2SO4 treated hydrolysates of corn stover. The most effective strain amongst the seven isolates was identified as Ochrobactrum ciceri W2. The W2 cells produced biopolymers in logarithm growth phase, peaking at 3.8 g l(-1)in productivity on 16 h. The yielded bioflocculant was primarily consisting of polysaccharides and proteins, and maintained its flocculating activity to 0.5% w/w kaolin suspensions over pH 1-10 (at 30°C) and 30-100°C (at pH 7). This study also revealed that the strain W2 could utilize biopolymers from hydrolysate of corn stover without addition of excess phosphate salts, which could largely reduce production costs of bioflocculants.
本研究从经 1.7%硫酸处理的玉米秸秆水解产物中分离出了总共 7 株能够产生生物絮凝剂的好氧活性污泥纯培养物。在这 7 个分离株中，最有效的菌株被鉴定为 Ochrobactrum ciceri W2。W2 细胞在对数生长期产生生物聚合物，在 16 小时时生产力达到 3.8 g l(-1)的峰值。所产生的生物絮凝剂主要由多糖和蛋白质组成，并在 pH 值为 1-10（在 30°C 下）和 30-100°C（在 pH 值为 7 下）时对 0.5% w/w 高岭土悬浮液保持絮凝活性。本研究还表明，菌株 W2 可以利用玉米秸秆水解物中的生物聚合物，而无需添加过量的磷酸盐，这可以大大降低生物絮凝剂的生产成本。
