Centre for Organismal Studies, Im Neuenheimer Feld 230, Heidelberg University, 69120 Heidelberg, Germany.
Dev Cell. 2012 Dec 11;23(6):1111-20. doi: 10.1016/j.devcel.2012.10.008.
It is a challenge in developmental biology to understand how an embryo's genes, proteins, and cells function and interact to govern morphogenesis, cell fate specification, and patterning. These processes span very different spatial and temporal scales. Despite much progress, simultaneous observation of such vastly differing scales has been beyond the scope of conventional microscopy. Light sheet microscopy fills this gap and is increasingly used for long-term, high-speed recordings of large specimens with high contrast and up to subcellular spatial resolution. We provide an overview of applications of light sheet microscopy in developmental biology and discuss future perspectives in this field.
在发育生物学中，理解胚胎的基因、蛋白质和细胞如何发挥功能并相互作用以控制形态发生、细胞命运特化和模式形成是一个挑战。这些过程跨越了非常不同的时空尺度。尽管已经取得了很大的进展，但传统显微镜的观察范围仍然无法同时观察到如此巨大的差异。光片显微镜填补了这一空白，并且越来越多地用于对具有高对比度和高达亚细胞空间分辨率的大样本进行长期、高速记录。我们提供了光片显微镜在发育生物学中的应用概述，并讨论了该领域的未来展望。
