Department of Urology, Medical University of Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria,
World J Urol. 2013 Dec;31(6):1389-94. doi: 10.1007/s00345-012-0997-8. Epub 2012 Dec 14.
To investigate the prevalence of high-risk (HR) and low-risk (LR) human papillomavirus (HPV) in prepuces of boys and men without any HPV related lesions.
Between 2009 and 2011, a total collective of 250 boys and men were investigated in this prospective study. The samples were subdivided into 3 groups regarding their age, consisting of 125 (50%) children (0-10 years), 38 (15.2%) adolescents (11-20 years) and 87 (34.8%) adults (>20 years). In situ hybridization (ISH) was performed to detect HR and LR virus types within the epithelium, followed by microscopic interpretation and determination between episomal and integrative signal pattern.
Our results revealed high levels of HPV concentration (HR and LR) in all age groups: HR versus LR positivity was seen in 45.5 versus 35% (children), 60.6 versus 63.6% (adolescents) and 58.3 versus 48.6% (adults). The topmost rate of HR (59.8%)- and LR (50.6%)-positive probes was found in the group with high estimated sexual activity (>14 years).
Concerning the high prevalence of male HPV infection even in children, indicating non-sexual transmission pathways, inclusion of boys in the vaccination program seems to be required reducing their burden of HPV related disease.
调查无 HPV 相关病变的男童和男性包皮中高危（HR）和低危（LR）人乳头瘤病毒（HPV）的流行情况。
在 2009 年至 2011 年期间，本前瞻性研究共调查了 250 名男童和男性。根据年龄将样本分为 3 组，包括 125 名（50%）儿童（0-10 岁）、38 名（15.2%）青少年（11-20 岁）和 87 名（34.8%）成年人（>20 岁）。采用原位杂交（ISH）检测上皮内 HR 和 LR 病毒类型，然后进行显微镜解释，并确定外显子和整合信号模式。
我们的结果显示所有年龄组 HPV 浓度（HR 和 LR）均较高：HR 与 LR 阳性率分别为 45.5%与 35%（儿童）、60.6%与 63.6%（青少年）和 58.3%与 48.6%（成年人）。在性活动估计较高（>14 岁）的组中，HR（59.8%）和 LR（50.6%）阳性探针的比例最高。
鉴于男童 HPV 感染率较高，甚至在儿童中也如此，提示存在非性传播途径，因此需要将男童纳入疫苗接种计划，以降低其 HPV 相关疾病负担。
