使用 CELL-DYN Sapphire 血液学分析仪分析浆体液。
Analysis of serous body fluids using the CELL-DYN Sapphire hematology analyzer.
机构信息
Department of Clinical Chemistry and Hematology, Atrium Medical Center, Heerlen, The Netherlands.
出版信息
Clin Chem Lab Med. 2013 Jun;51(6):1285-90. doi: 10.1515/cclm-2012-0549.
BACKGROUND
Correct cell enumeration and differential analysis of body fluids are important in the diagnosis and management of several diseases. Currently, microscopic analysis is still considered the "gold standard". The aim of the present study was to evaluate the analytical performance of the CELL-DYN Sapphire hematology analyzer for automated differentiation of cells in serous fluids and to explore whether manual analysis of the raw data files could improve the differential count compared with reference microscopy.
METHODS
A total of 105 serous fluids (39 peritoneal and 66 pleural effusions) were analyzed by the CELL-DYN Sapphire using standard whole-blood algorithm. Additionally, we performed optimized manual gating of the Sapphire raw data file using standard flow cytometry software.
RESULTS
The standard Sapphire algorithm showed substantial deviations from the reference microscopic differentiation: polymorphonuclear cell counts were too high because they contained some monocytic cells. However, when optimized manual gating strategy is used, a good correlation and negligible bias were found.
CONCLUSIONS
We have demonstrated that with a modified algorithm, CELL-DYN Sapphire will provide reliable identification and enumeration of blood cells in peritoneal and pleural fluids.
背景
正确的细胞计数和体液的差异分析在几种疾病的诊断和治疗中非常重要。目前，显微镜分析仍然被认为是“金标准”。本研究的目的是评估 CELL-DYN Sapphire 血液分析仪在自动区分浆膜液细胞方面的分析性能，并探讨与参考显微镜相比，手动分析原始数据文件是否可以提高差异计数。
方法
使用标准全血算法对 105 份浆膜液（39 份腹腔液和 66 份胸腔积液）进行了 CELL-DYN Sapphire 分析。此外，我们使用标准流式细胞术软件对 Sapphire 原始数据文件进行了优化的手动门控。
结果
标准 Sapphire 算法与参考显微镜的分化存在显著偏差：多形核细胞计数过高，因为其中含有一些单核细胞。然而，当使用优化的手动门控策略时，发现了良好的相关性和可忽略的偏差。
结论
我们已经证明，通过修改算法，CELL-DYN Sapphire 将为腹腔和胸腔液中的血液细胞提供可靠的识别和计数。
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