Risquez F, Boyer P, Rolet F, Magnani M, Guichard A, Cedard L, Zorn J R
Centre de PMA Baudelocque-St Vincent de Paul, Paris, France.
Hum Reprod. 1990 Feb;5(2):185-8. doi: 10.1093/oxfordjournals.humrep.a137066.
This preliminary study was designed to evaluate retrograde cannulation of the Fallopian tubes up to the isthmo-interstitial junction using the new technique of tubal embryo stage transfer (TEST). Follicular aspiration was performed under the guidance of a vaginal ultrasound probe in 51 women treated with GnRH + HMG. The oocytes retrieved were inseminated in vitro with 50,000 motile spermatozoa and kept in Menezo B2 medium without serum, at 37 degrees C, in an atmosphere of air + 5% CO2. The eggs were checked 24 and 36 h after insemination. No fertilization occurred in 23 patients. Cleaved embryos were obtained in the 28 other patients. One to seven embryos at the 2-4-cell stage were transferred with the 'Baudelocque Black Catheter' (BBC) into one tube and spare embryos were frozen. Five pregnancies occurred after retrograde TEST, for a pregnancy rate of 9.8% per cycle and 17.9% per transfer. One patient has given birth to a normal full-term baby. One singleton and one twin pregnancy are ongoing (8 months in June 1989). The other two pregnancies were ectopic.
本初步研究旨在使用输卵管胚胎期移植（TEST）新技术评估输卵管逆行插管直至峡部-间质部交界处的情况。在51例接受促性腺激素释放激素（GnRH）+人绝经期促性腺激素（HMG）治疗的妇女中，在阴道超声探头引导下进行卵泡抽吸。获取的卵母细胞与50000个活动精子进行体外受精，并置于不含血清的Menezo B2培养基中，于37℃、空气+5%二氧化碳的环境中培养。授精后24小时和36小时检查卵子。23例患者未发生受精。其他28例患者获得了分裂胚。将1至7个处于2-4细胞期的胚胎用“巴德洛克黑猫导管”（BBC）移植到一侧输卵管中，剩余胚胎冷冻保存。逆行TEST后发生了5次妊娠，每个周期的妊娠率为9.8%，每次移植的妊娠率为17.9%。1例患者已分娩出一名正常足月婴儿。1例单胎妊娠和1例双胎妊娠仍在继续（1989年6月为8个月）。另外2例妊娠为异位妊娠。
