Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Nucleic Acids Res. 2013 Feb 1;41(3):1797-806. doi: 10.1093/nar/gks1335. Epub 2012 Dec 20.
RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.
RNA 聚合酶 II（Pol II）的终止既依赖于 RNA 加工信号，也依赖于位于多聚（A）位点下游的特定终止元件。到目前为止，描述的两个主要终止子类别之一是共转录切割（CoTC）元件。我们表明，人类β-珠蛋白 CoTC 介导的终止子元件中的同聚体 A/T 片段在 Pol II 终止中起着关键作用。这些散布在看似随机序列中的短 A/T 片段是强终止子元件，生物信息学分析证实，在全基因组的 70%的假定终止区（PTR）中存在这样的序列。
