Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE Oxford, UK.
Cell Rep. 2013 Apr 25;3(4):1080-92. doi: 10.1016/j.celrep.2013.03.012. Epub 2013 Apr 4.
Mammalian RNA polymerase II (Pol II) transcription termination is an essential step in protein-coding gene expression that is mediated by pre-mRNA processing activities and DNA-encoded terminator elements. Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited. Whereas promoter databases list up to 40,000 known and potential Pol II promoter sequences, fewer than ten Pol II terminator sequences have been described. Using our knowledge of the human β-globin terminator mechanism, we have developed a selection strategy for mapping mammalian Pol II terminator elements. We report the identification of 78 cotranscriptional cleavage (CoTC)-type terminator elements at endogenous gene loci. The results of this analysis pave the way for the full understanding of Pol II termination pathways and their roles in gene expression.
哺乳动物 RNA 聚合酶 II（Pol II）转录终止是蛋白质编码基因表达的一个必要步骤，由前体 mRNA 加工活性和 DNA 编码的终止元件介导。尽管人们对前体 mRNA 加工在终止中的作用有了很多了解，但我们对终止元件的特征和普遍性的理解是有限的。虽然启动子数据库列出了多达 40,000 个已知和潜在的 Pol II 启动子序列，但描述的 Pol II 终止子序列不到十个。利用我们对人类β-珠蛋白终止子机制的了解，我们开发了一种用于绘制哺乳动物 Pol II 终止子元件的选择策略。我们报告了在内源性基因位点鉴定了 78 个共转录切割（CoTC）型终止子元件。该分析的结果为全面了解 Pol II 终止途径及其在基因表达中的作用铺平了道路。
