Korean Medicine (KM)-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:985151. doi: 10.1155/2012/985151. Epub 2012 Nov 29.
Porcine epidemic diarrhea virus (PEDV) causes diarrhea of pigs age-independently and death of young piglets, resulting in economic loss of porcine industry. We have screened 333 natural oriental herbal medicines to search for new antiviral candidates against PEDV. We found that two herbal extracts, KIOM 198 and KIOM 124, contain significant anti-PED viral effect. KIOM 198 and KIOM 124 were identified as Epimedium koreanum Nakai and Lonicera japonica Thunberg, respectively. The further plaque and CPE inhibition assay in vitro showed that KIOM 198 has much stronger antiviral activity than KIOM 124. Additionally, KIOM 198 exhibited a similar extent of antiviral effect against other subtypes of Corona virus such as sm98 and TGE viruses. Cytotoxicity results showed that KIOM 198 is nontoxic on the cells and suggest that it can be delivered safely for therapy. Furthermore, when we orally administered KIOM 198 to piglets and then infected them with PEDV, the piglets did not show any disease symptoms like diarrhea and biopsy results showed clean intestine, whereas control pigs without KIOM 198 treatment exhibited PED-related severe symptoms. These results imply that KIOM 198 contains strong antiviral activity and has a potential to be developed as an antiviral phytomedicine to treat PEDV-related diseases in pigs.
猪流行性腹泻病毒（PEDV）可引起各年龄段猪只发生腹泻，导致仔猪死亡，给养猪业带来严重的经济损失。我们筛选了 333 种天然东方草药，以寻找针对 PEDV 的新型抗病毒候选药物。我们发现两种草药提取物 KIOM 198 和 KIOM 124 具有显著的抗 PEDV 作用。KIOM 198 和 KIOM 124 分别被鉴定为朝鲜淫羊藿和忍冬。进一步的体外蚀斑和 CPE 抑制试验表明，KIOM 198 的抗病毒活性明显强于 KIOM 124。此外，KIOM 198 对其他冠状病毒亚属，如 sm98 和 TGE 病毒也具有相似程度的抗病毒作用。细胞毒性结果表明 KIOM 198 对细胞无毒，提示其可安全用于治疗。此外，当我们给仔猪口服 KIOM 198 然后用 PEDV 感染它们时，仔猪没有出现任何腹泻等疾病症状，活检结果显示肠道清洁，而没有接受 KIOM 198 治疗的对照猪则表现出与 PED 相关的严重症状。这些结果表明 KIOM 198 具有较强的抗病毒活性，有望开发成为治疗猪 PEDV 相关疾病的抗病毒植物药。
