Peng Ju-Yi, Horng Yi-Bing, Wu Ching-Ho, Chang Chia-Yu, Chang Yen-Chen, Tsai Pei-Shiue, Jeng Chian-Ren, Cheng Yeong-Hsiang, Chang Hui-Wen
Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, No. 1, Section 4, Roosevelt Rd., Taipei, 10617, Taiwan.
School of Veterinary Medicine, National Taiwan University, No. 1, Section 4, Roosevelt Rd., Taipei, 10617, Taiwan.
AMB Express. 2019 Dec 3;9(1):191. doi: 10.1186/s13568-019-0916-0.
Bacillus licheniformis (B. licheniformis) is commonly used as probiotic and its secondary metabolites are attractive anti-microbial candidate. In the present study, we aimed to evaluate the antiviral activity of crude extracts from B. licheniformis against porcine epidemic diarrhea virus (PEDV), a highly contagious enveloped porcine virus that has caused great economic loss in pigs. In vivo, PEDV-infected piglets supplemented with air-dried solid state fermentative cultivate containing B. licheniformis-fermented products (BLFP) showed milder clinical symptoms and decreased viral shedding. Importantly, no significant systemic pathological lesions and no reduction in average daily gain were noted in pigs supplemented with the BLFP, which suggests that it is safe for use in pigs. In vitro experiments revealed that while B. licheniformis crude extracts exhibited no toxicity in Vero cells, co-cultivation of B. licheniformis crude extracts with PEDV significantly reduced viral infection and replication. Summarized current results suggest that the B. licheniformis-fermented products could be a novel candidate food additive for reducing the impact of PED on the swine industry.
地衣芽孢杆菌通常用作益生菌，其次级代谢产物是有吸引力的抗菌候选物。在本研究中，我们旨在评估地衣芽孢杆菌粗提物对猪流行性腹泻病毒（PEDV）的抗病毒活性，PEDV是一种高度传染性的有包膜猪病毒，已给养猪业造成巨大经济损失。在体内，用含有地衣芽孢杆菌发酵产物（BLFP）的风干固态发酵培养物补充感染PEDV的仔猪，其临床症状较轻，病毒脱落减少。重要的是，补充BLFP的猪未观察到明显的全身病理损伤，平均日增重也未降低，这表明其在猪中使用是安全的。体外实验表明，虽然地衣芽孢杆菌粗提物在Vero细胞中无毒性，但地衣芽孢杆菌粗提物与PEDV共培养可显著降低病毒感染和复制。总结目前的结果表明，地衣芽孢杆菌发酵产物可能是一种新型的食品添加剂，可减少PED对养猪业的影响。
