Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, United States.
Anal Chim Acta. 2013 Jan 8;759:74-80. doi: 10.1016/j.aca.2012.10.038. Epub 2012 Nov 12.
Vasopressin is an indicating biomarker for blood pressure in the human body and low vasopressin levels can be indicative of late-phase hemorrhagic shock or other traumatic injuries. In this paper we have developed an aptamer-based label-free microfluidic biosensor for the electrochemical detection of vasopressin. The detection area consists of aptamers immobilized on carbon nanotubes which specifically capture the vasopressin molecules in solution resulting in changes in conductivity across the sensor. We report a limit of detection of 43 pM in standard solutions and demonstrate high detection specificity toward vasopressin when different interferents are present. The miniaturized microfluidic biosensor offers continuous monitoring of different vasopressin levels with good potential for portability. Ultimately such a system could serve as a point-of-care diagnostics tool for patients with excessive bleeding when standard medical infrastructure is not available.
血管加压素是人体血压的指示性生物标志物，血管加压素水平低可能表明处于晚期失血性休克或其他创伤性损伤。在本文中，我们开发了一种基于适体的无标记微流控生物传感器，用于血管加压素的电化学检测。检测区域由固定在碳纳米管上的适体组成，这些适体特异性地捕获溶液中的血管加压素分子，从而导致传感器的电导率发生变化。我们报告了在标准溶液中的检测限为 43 pM，并证明了当存在不同干扰物时，该传感器对血管加压素有很高的检测特异性。这种小型化的微流控生物传感器可以连续监测不同水平的血管加压素，具有很好的便携性潜力。最终，当标准医疗基础设施不可用时，这样的系统可以作为大量出血患者的即时诊断工具。
