Zelazowska-Rutkowska Beata, Ilendo Elzbieta, Skotnicka Bozena, Wysocka Jolanta, Kasprzycka Edwina
Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2012;50(4):586-9. doi: 10.5603/20326.
Hypertrophic adenoids with otitis media with effusion is a common infectious disease and present a serious otological problem in children. Cytokines, potent inflammatory mediators, play important role in the initiation of immunological response in otitis media. Adenoids excised due to hypertrophy with or without chronic otitis media with effusion were used to isolate mononuclear cells. Secretion of cytokines by non-stimulated and PHA-stimulated cells was determined by specific ELISAs. We found a significant increase in the production of IL-5 and TNF-α secreted by adenoidal cells of children with otitis media with effusion compared to group with hypertrophic adenoids. No differences were found in the secretion of IL-8, IL-6, and IL-10 between these two groups of patients. Our results suggest a difference between the immunological responses in the course of hypertrophic adenoids with otitis media as compared to hypertrophic adenoids.
腺样体肥大伴分泌性中耳炎是一种常见的感染性疾病，也是儿童严重的耳科问题。细胞因子作为强效炎症介质，在中耳炎免疫反应的启动中起重要作用。因肥大而切除的腺样体，无论有无慢性分泌性中耳炎，均用于分离单核细胞。通过特异性酶联免疫吸附测定法（ELISA）测定未刺激和PHA刺激细胞的细胞因子分泌情况。我们发现，与腺样体肥大组相比，分泌性中耳炎患儿腺样体细胞分泌的IL-5和TNF-α显著增加。两组患者在IL-8、IL-6和IL-10的分泌方面未发现差异。我们的结果表明，与腺样体肥大相比，腺样体肥大伴中耳炎过程中的免疫反应存在差异。
