Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON, M5S 3H6, Canada.
Chem Phys Lipids. 2013 Jan;166:31-44. doi: 10.1016/j.chemphyslip.2012.12.004. Epub 2012 Dec 26.
Lateral diffusion is a fundamental property of biological membrane components, important for a host of biomembrane functions. Although long studied, novel aspects of the relationship between the structure of membrane components and their lateral diffusion properties continue to emerge. NMR-based lateral diffusion measurements are complicated by the spectral broadening arising from the slow anisotropic motions in membranes. Nevertheless, both pulsed field gradient (PFG) and exchange spectroscopy (EXSY) methods can be adapted to permit NMR measurements of lateral diffusion in membranes. These variously will be described in overview, highlighting advantages and limitations of each, but with particular emphasis on results from our laboratory using (1)H PFG NMR measurements in magnetically aligned bicelles and (31)P CODEX (Centreband-Only-Detection-of-Exchange) measurements in spherical phospholipid vesicles.
侧向扩散是生物膜成分的基本特性，对多种生物膜功能都很重要。尽管侧向扩散已经研究了很长时间，但膜成分的结构与其侧向扩散性质之间的关系仍不断有新的方面显现出来。基于 NMR 的侧向扩散测量受到膜中缓慢各向异性运动引起的光谱展宽的影响。尽管如此，脉冲梯度（PFG）和交换光谱（EXSY）方法都可以进行调整，以允许在膜中进行 NMR 侧向扩散测量。本文将对这些方法进行概述，重点介绍每种方法的优缺点，但特别强调我们实验室使用（1）H PFG NMR 在磁定向双胶束和（31）P CODEX（仅中心带检测交换）在球形磷脂囊泡中的测量结果。
