Cancer Metastasis Alert and Prevention Institute, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China.
Trends Biotechnol. 2013 Feb;31(2):99-107. doi: 10.1016/j.tibtech.2012.11.010. Epub 2012 Dec 30.
The complexity of proteomics challenges current methods to provide all peptide mass fingerprints in an ensemble measurement of various proteins at differing concentrations. To detect those low-abundance proteins, nanotechnology provides a technical platform to improve biocompatibility, specificity, reproducibility, and robustness of the current proteomic methods. Here, we comprehensively analyze the weaknesses of traditional proteomic methods and evaluate the importance of nanomaterials in significantly improving the quality of proteomic methods by manipulating individual proteins. We also illustrate how the large surface-to-volume ratio of nanomaterials can facilitate mass transfer, enhance the efficiency of separation and high-throughput capability, and reduce assay time and sample consumption. The marriage of the two subjects and the resulting new nanoproteomics will revolutionize proteomics research.
蛋白质组学的复杂性挑战了当前的方法，无法在对不同浓度的各种蛋白质进行的综合测量中提供所有肽质量指纹图谱。为了检测那些低丰度的蛋白质，纳米技术提供了一个技术平台，以提高当前蛋白质组学方法的生物相容性、特异性、重现性和稳健性。在这里，我们全面分析了传统蛋白质组学方法的弱点，并评估了纳米材料在通过操纵单个蛋白质显著提高蛋白质组学方法质量方面的重要性。我们还说明了纳米材料的大表面积与体积比如何促进质量传递、提高分离和高通量能力，并减少分析时间和样品消耗。这两个学科的结合以及由此产生的新的纳米蛋白质组学将彻底改变蛋白质组学研究。
