Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, Tunis, Tunisia.
Vector Borne Zoonotic Dis. 2013 Feb;13(2):98-102. doi: 10.1089/vbz.2012.1047. Epub 2013 Jan 5.
Our objective was to analyze the carriage rate of extended-spectrum β-lactamase (ESBL)- and plasmidic AmpC β-lactamase (pAmpC)-producing Escherichia coli isolates in fecal samples of healthy pets (dogs and cats) and to characterize the recovered isolates for the presence of other resistance genes and integrons. Eighty fecal samples of healthy pets were inoculated in MacConkey agar plates supplemented with cefotaxime (2 μg/mL) for cefotaxime-resistant (CTX(R)) E. coli recovery. CTX(R) E. coli isolates were detected in 14 of the 80 fecal samples (17.5%) and the following β-lactamase genes (number of isolates) were detected: bla(CTX-M-1) (8), bla(CTX-M-1)+bla(TEM-1b) (3)(,) bla(CTX-M-1)+bla(TEM-1c) (1), bla(CTX-M-1)+bla(TEM-135) (1), and bla(CMY-2)+bla(TEM-1b) (1). The 14 E. coli were distributed into the phylogroups B1 (6 isolates), A (5), and D (3). The qnrB19 gene was detected in one CTX-M-1-producing strain of phylogroup D. Five isolates contained class 1 integrons with the following arrangements: dfrA17-aadA5 (2 isolates), dfrA1-aadA1 (1), and dfrA17-aadA5/ dfrA1-aadA1 (2 isolates). The virulence genes fimA and/or aer were detected in all CTX(R) strains. In this study, the pet population harbored β-lactamase and quinolone resistance genes of special interest in human health that potentially could be transmitted to humans in close contact with them.
我们的目的是分析健康宠物（狗和猫）粪便样本中携带产超广谱β-内酰胺酶（ESBL）和质粒型 AmpCβ-内酰胺酶（pAmpC）的大肠杆菌的携带率，并对分离出的菌株进行其他耐药基因和整合子的特征分析。将 80 份健康宠物粪便样本接种于添加头孢噻肟（2μg/mL）的麦康凯琼脂平板上，以回收头孢噻肟耐药（CTX(R)）大肠杆菌。在 80 份粪便样本中检出 14 份（17.5%）CTX(R)大肠杆菌，检测到以下β-内酰胺酶基因（分离株数）：bla(CTX-M-1)（8），bla(CTX-M-1)+bla(TEM-1b)（3），bla(CTX-M-1)+bla(TEM-1c)（1），bla(CTX-M-1)+bla(TEM-135)（1），和 bla(CMY-2)+bla(TEM-1b)（1）。14 株大肠杆菌分布于 B1 组（6 株）、A 组（5 株）和 D 组（3 株）。CTX-M-1 阳性的 D 组菌株中检测到 qnrB19 基因。5 株菌携带的 1 类整合子，排列方式为：dfrA17-aadA5（2 株）、dfrA1-aadA1（1 株）和 dfrA17-aadA5/dfrA1-aadA1（2 株）。所有 CTX(R)菌株均检测到 fimA 和/或 aer 毒力基因。在这项研究中，宠物群体携带了对人类健康具有特殊意义的β-内酰胺酶和喹诺酮类耐药基因，这些基因可能会通过与它们密切接触而传播给人类。
