肥胖和正常体重不孕妇女来源的胚胎具有相似的形态动力学模式：一项时间延迟研究。
Similar morphokinetic patterns in embryos derived from obese and normoweight infertile women: a time-lapse study.
机构信息
Instituto Valenciano de Infertilidad, University of Valencia, Valencia, Spain.
出版信息
Hum Reprod. 2013 Mar;28(3):794-800. doi: 10.1093/humrep/des438. Epub 2013 Jan 4.
STUDY QUESTION
Does female obesity affect the dynamic parameters of embryo quality assessed by time-lapse analysis?
SUMMARY ANSWER
Female obesity does not affect the dynamic embryo quality as determined by image acquisition and time-lapse analysis.
WHAT IS KNOWN ALREADY
Female obesity impairs natural and assisted reproduction but there is no agreement on the specific contribution of gametes, embryos or endometrial receptivity. In this preliminary study the dynamic parameters of embryo quality are assessed for the first time by time-lapse analysis.
STUDY DESIGN, SIZE, DURATION: Two-year cohort retrospective study comparing embryos from three groups of patients according to the presence of infertility and/or obesity.
PARTICIPANTS AND SETTING
Participants attended a University-affiliated private clinic where ICSI was performed. Using an IVF incubator with a built-in camera designed to automatically acquire images at defined time points, we monitored individual embryos from 89 patients: 71 embryos from 13 obese infertile women, 242 embryos from 45 normoweight infertile women and 111 embryos from 31 normoweight fertile oocyte donors. The chronological pattern of cell divisions (timings of cell cleavages) and other morphologic features (time-dependent cell size and nucleation) was recorded.
MAIN RESULTS AND THE ROLE OF CHANCE
Embryos from obese and normoweight infertile women showed similar cleavage patterns, but occurring more slowly, to those from fertile donors. These differences were statistically significant for t2 (time of cleavage to two-blastomere embryo) (P = 0.016), t3 (P = 0.014), t4 (P = 0.003) and t5 (P = 0.040).
LIMITATIONS, REASONS FOR CAUTION: These are preliminary data from a retrospective analysis with a limited sample size.
GENERALIZABILITY TO OTHER POPULATIONS
Not recommended until further studies using time-lapse analysis of a larger sample have been performed.
STUDY FUNDING/COMPETING INTEREST(S): None.
研究问题
肥胖女性的体脂是否会影响通过时差分析评估的胚胎活力的动态参数？
总结答案
肥胖女性的体脂不会影响胚胎活力，这是通过图像采集和时差分析确定的。
已知信息
肥胖会损害自然和辅助生殖，但对于配子、胚胎或子宫内膜容受性的具体贡献尚无共识。在这项初步研究中，首次通过时差分析评估了胚胎活力的动态参数。
研究设计、规模、持续时间：这是一项为期两年的队列回顾性研究，比较了根据不孕和/或肥胖情况分为三组患者的胚胎。
参与者和设置
参与者在一所大学附属的私人诊所就诊，在那里进行了 ICSI。我们使用带有内置摄像头的 IVF 孵育器，该摄像头设计用于在定义的时间点自动获取图像，监测了 89 名患者的 111 个胚胎：13 名肥胖不孕妇女的 71 个胚胎、45 名体重正常不孕妇女的 242 个胚胎和 31 名体重正常有生育能力的卵母细胞供体的 111 个胚胎。记录了细胞分裂的时间顺序（细胞分裂的时间）和其他形态特征（随时间变化的细胞大小和核形成）。
主要结果及其机会作用
肥胖和体重正常的不孕妇女的胚胎显示出与生育力供体相似的分裂模式，但速度较慢。对于 t2（两个胚胎分裂时间）（P = 0.016）、t3（P = 0.014）、t4（P = 0.003）和 t5（P = 0.040），这些差异具有统计学意义。
局限性、谨慎的原因：这些是初步数据，来自具有有限样本量的回顾性分析。
在其他人群中的推广性
在进行了更大样本量的时差分析研究之前，不建议推广。
研究资金/竞争利益：无。
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