Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA.
J Fish Dis. 2013 Jun;36(6):533-42. doi: 10.1111/jfd.12044. Epub 2013 Jan 7.
There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty-four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future.
人们越来越关注观赏鱼养殖业中超量使用抗生素的问题，以及这可能对这些鱼类体内共生菌和病原菌的耐药性不断增加产生的影响。本研究旨在对进口到北美的观赏鱼物种中的细菌多样性（包括病原体）进行评估，并评估其抗生素耐药性。从来自哥伦比亚、新加坡和佛罗里达的进口设施抵达俄勒冈州波特兰的 32 种淡水观赏鱼的肾脏样本中采集了样本。使用细菌 16S 引物和 DNA 测序技术，通过 PCR 从 64 个独特的细菌菌落中分离并鉴定出这些细菌。多个分离株被鉴定为具有在鱼类和人类中引起疾病潜力的细菌。对每个分离株的抗生素耐药谱进行了 9 种不同抗生素的检测。其中，头孢噻肟（16%的分离株耐药）是活性最强的抗生素，而活性最弱的是四环素（77%的分离株耐药）。了解进口观赏鱼中细菌的多样性信息以及这些细菌的耐药谱，将有助于更有效地治疗临床感染的鱼类，并为未来可能出现的人畜共患病提供参考。
