Department of Environmental Microbiology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-957 Olsztyn, Poland.
Department of Environmental Microbiology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-957 Olsztyn, Poland.
Chemosphere. 2015 Jun;128:134-41. doi: 10.1016/j.chemosphere.2015.01.035. Epub 2015 Feb 17.
The aim of this study was to assess the impact of a fish farm on the structure of antibiotic resistant bacteria and antibiotic resistance genes in water of Drwęca River. Samples of upstream river waters; post-production waters and treated post-production waters from fish farm; as well as downstream river waters were monitored for tetracycline resistant bacteria, tetracycline resistant genes, basic physico-chemical parameters and tetracyclines concentration. The river waters was characterized by low levels of pollution, which was determined based on water temperature, pH and concentrations of dissolved oxygen and tetracycline antibiotics. Culture-dependent (heterotrophic plate counts, counts of bacteria resistant to oxytetracycline (OTC(R)) and doxycycline (DOX(R)), minimum inhibitory concentrations for oxytetracycline and doxycycline, multidrug resistance of OTC(R) and DOX(R), qualitative composition of OTC(R) and DOX(R), prevalence of tet genes in resistant isolates) and culture-independent surveys (quantity of tet gene copies) revealed no significant differences in the abundance of antibiotic-resistant bacteria and antibiotic resistance genes between the studied samples. The only way in which the fish farm influenced water quality in the Drwęca River was by increasing the diversity of tetracycline-resistance genes. However, it should also be noted that the bacteria of the genera Aeromonas sp. and Acinetobacter sp. were able to transfer 6 out of 13 tested tet genes into Escherichiacoli, which can promote the spread of antibiotic resistance in the environment.
本研究旨在评估鱼类养殖场对德雷韦卡河水中抗生素耐药菌和抗生素耐药基因结构的影响。监测了上游河水、养殖场生产后的水和处理后的生产后水以及下游河水，以检测四环素耐药菌、四环素耐药基因、基本理化参数和四环素浓度。根据水温和 pH 值以及溶解氧和四环素抗生素的浓度，河水的污染程度较低。基于传统培养方法（异养平板计数、对土霉素（OTC(R)）和强力霉素（DOX(R)）耐药的细菌计数、土霉素和强力霉素的最小抑菌浓度、OTC(R)和 DOX(R)的多药耐药性、OTC(R)和 DOX(R)的定性组成、耐药分离株中的 tet 基因的流行率）和非培养方法（tet 基因拷贝数）的调查结果表明，研究样本中的抗生素耐药菌和抗生素耐药基因的丰度没有显著差异。鱼类养殖场对德雷韦卡河水质的唯一影响是增加了四环素耐药基因的多样性。然而，还应该注意到，气单胞菌属和不动杆菌属的细菌能够将 13 个测试的 tet 基因中的 6 个转移到大肠杆菌中，这可能会促进环境中抗生素耐药性的传播。
