CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Mar Environ Res. 2013 Mar;84:51-9. doi: 10.1016/j.marenvres.2012.11.009. Epub 2012 Dec 27.
Though there is some information on cytotoxicity of copper nanoparticles and silver nanoparticles on human cell lines, there is no information on their genotoxic and cytotoxic behaviour in bivalve molluscs. The aim of this study was to investigate the genotoxic impact of copper oxide and silver nanoparticles using mussels Mytilus galloprovincialis. Mussels were exposed to 10 μg L⁻¹ of CuO nanoparticles and Cu²⁺ and Ag nanoparticles and Ag⁺ for 15 days to assess genotoxic effects in hemocytes using the comet assay. The results obtained indicated that copper and silver forms (nanoparticles and ionic) induced DNA damage in hemolymph cells and a time-response effect was evident when compared to unexposed mussels. Ionic forms presented higher genotoxicity than nanoparticles, suggesting different mechanisms of action that may be mediated through oxidative stress. DNA strand breaks proved to be a useful biomarker of exposure to genotoxic effects of CuO and Ag nanoparticles in marine molluscs.
虽然有一些关于铜纳米粒子和银纳米粒子对人细胞系的细胞毒性的信息，但关于它们在双壳类软体动物中的遗传毒性和细胞毒性行为的信息却很少。本研究旨在使用贻贝 Mytilus galloprovincialis 来研究氧化铜和银纳米粒子的遗传毒性影响。贻贝暴露于 10 μg L⁻¹的 CuO 纳米粒子和 Cu²⁺以及 Ag 纳米粒子和 Ag⁺中 15 天，以使用彗星试验评估血细胞中的遗传毒性效应。结果表明，铜和银的形式（纳米粒子和离子）在血液细胞中诱导了 DNA 损伤，与未暴露的贻贝相比，表现出明显的时间反应效应。离子形式表现出更高的遗传毒性，这表明可能通过氧化应激介导不同的作用机制。DNA 链断裂被证明是一种有用的生物标志物，可用于暴露于海洋软体动物中的 CuO 和 Ag 纳米粒子的遗传毒性效应。
