Viacheslavova A O, Berdichevets I N, Tiurin A A, Shimshilashvili Kh R, Mustafaev O, Goldenkova-Pavlova I V
Genetika. 2012 Nov;48(11):1245-59.
The basic methods used in current practice for stable and transient expression of heterologous genes in plants are presented and compared. The key areas of research in the heterologous expression of genes in plants have been identified by analyzing literature and experimental data: modeling of metabolic pathways; creation of marker-free transgenic plants; the search for new regulatory elements and plant genes influencing the efficiency of expression of heterologous genes in plants; development of new methods for analyzing of transgenic plants and new approaches to the expression of heterologous genes in plants.
本文介绍并比较了当前在植物中稳定和瞬时表达异源基因的常用基本方法。通过分析文献和实验数据，确定了植物异源基因表达研究的关键领域：代谢途径建模；无标记转基因植物的创建；寻找影响植物中异源基因表达效率的新调控元件和植物基因；开发分析转基因植物的新方法以及植物中异源基因表达的新途径。
