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与大豆再生相关的GmESR1基因的功能分析

Functional analysis of the GmESR1 gene associated with soybean regeneration.

作者信息

Zhang Chao, Wu Xiaodong, Zhang Binbin, Chen Qingshan, Liu Ming, Xin Dawei, Qi Zhaoming, Li Sinan, Ma Yanlong, Wang Lingshuang, Jin Yangmei, Li Wenbin, Wu Xiaoxia, Su An-Yu

机构信息

Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, Heilongjiang province, People's Republic of China.

College of Resources and Environment, Northeast Agricultural University. Harbin, Heilongjiang province, People's Republic of China.

出版信息

PLoS One. 2017 Apr 12;12(4):e0175656. doi: 10.1371/journal.pone.0175656. eCollection 2017.

DOI:10.1371/journal.pone.0175656
PMID:28403182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5389854/
Abstract

Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar 'Dongnong 50'. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean.

摘要

植物再生可以通过体细胞胚胎发生或从头芽器官发生的体外组织培养来实现。大豆（Glycine max）的转化较为困难，因此需要优化大豆再生的转化系统。本研究调查了芽再生增强子1（GmESR1），这是一种靶向再生相关基因的大豆转录因子。序列分析表明，GmESR1包含一个保守的57个氨基酸的APETALA 2（AP2）/乙烯响应因子（ERF）DNA结合结构域。在大豆品种‘东农50’中，GmESR1的相对表达水平在幼胚、花和茎中最高。为了研究GmESR1的功能，构建了过表达GmESR1的转基因拟南芥（Arabidopsis thaliana）和大豆植株。在拟南芥中，GmESR1的过表达导致种子萌发加速，幼苗芽和根伸长。在大豆中，GmESR1的过表达也导致种子萌发加快，芽和根伸长。GmESR1特异性结合GCC盒。这些结果为建立高效稳定的大豆转化系统奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/a6c1f593ece9/pone.0175656.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/a16216be9fb7/pone.0175656.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/85492dd8dd8c/pone.0175656.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/e9c47523c3cd/pone.0175656.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/0fad9f2ec295/pone.0175656.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/454c3b71bbb2/pone.0175656.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/6583811de526/pone.0175656.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/a6c1f593ece9/pone.0175656.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/a16216be9fb7/pone.0175656.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/85492dd8dd8c/pone.0175656.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/e9c47523c3cd/pone.0175656.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/0fad9f2ec295/pone.0175656.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/454c3b71bbb2/pone.0175656.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/6583811de526/pone.0175656.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb7/5389854/a6c1f593ece9/pone.0175656.g007.jpg

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