Laboratoire Antibiotiques Antifongiques: Physico-Chimie, Synthèse et Activité Biologique, Université Abou Bekr Belkaïd, Tlemcen, Algérie.
Microb Drug Resist. 2013 Jun;19(3):185-90. doi: 10.1089/mdr.2012.0161. Epub 2013 Jan 9.
Seventy-one isolates of Enterobacteriaceae (17 Escherichia coli, 50 Klebsiella pneumoniae, and 4 Enterobacter cloacae) producing extended-spectrum-β-lactamases (ESBLs) were collected between April 2008 and March 2010 in an intensive care unit and surgical ward of Tlemcen Hospital (West of Algeria). Sequencing identified the bla(CTX-M-15) determinant in 69 isolates and bla(CTX-M-3) in 2 isolates. None of the studied strains produced the class D carbapenemase OXA-48. Repetitive Extragenic palindromic polymerase chain reaction showed a high degree of genotypic diversity among E. coli strains and two major clonal populations of K. pneumoniae (CKp1 n=11 and CKp5 n=25) which were further identified as members of the multilocus sequence typing types (ST931) and (ST15), respectively. The ST15 isolates harbored more resistance genes and virulence factors than the ST931 isolates. The characterization of the spacer region between ISEcp1 and bla(CTX-M) for CTX-M-15 producers individualized two populations. One that derived from the CTX-M-3 under Algerian clinical context and one that is universally found. The dissemination of ESBLs in the studied Enterobacteriaceae isolates was mainly due to the epidemic clones of K. pneumoniae and to genetic transit of plasmids among unrelated strains.
2008 年 4 月至 2010 年 3 月期间，在阿尔及利亚西部特莱姆森医院的重症监护病房和外科病房中收集了 71 株产生超广谱β-内酰胺酶（ESBL）的肠杆菌科（17 株大肠埃希菌、50 株肺炎克雷伯菌和 4 株阴沟肠杆菌）。测序确定了 69 株 bla(CTX-M-15)决定簇和 2 株 bla(CTX-M-3)。研究的菌株均不产生类 D 碳青霉烯酶 OXA-48。重复外显子回文聚合酶链反应显示大肠埃希菌菌株具有高度的基因型多样性，肺炎克雷伯菌有两个主要的克隆种群（CKp1 n=11 和 CKp5 n=25），进一步鉴定为多位点序列分型（ST931）和（ST15）的成员。ST15 分离株比 ST931 分离株携带更多的耐药基因和毒力因子。CTX-M-15 产生菌中 ISEcp1 和 bla(CTX-M)之间间隔区的特征表明存在两个群体。一个源于阿尔及利亚临床背景下的 CTX-M-3，另一个则是普遍存在的。在所研究的肠杆菌科分离株中，ESBL 的传播主要是由于肺炎克雷伯菌的流行克隆和质粒在无关菌株之间的遗传转移。
