Ayad Amel, Drissi Mourad, de Curraize Claire, Dupont Chloé, Hartmann Alain, Solanas Sébastien, Siebor Eliane, Amoureux Lucie, Neuwirth Catherine
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, DijonFrance; Laboratoire Antibiotiques, Antifongiques: Physico-chimie, Synthèse et Activité Biologique, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid, TlemcenAlgérie; Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université Hassiba Benbouali, ChlefAlgérie.
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid, Tlemcen Algérie.
Front Microbiol. 2016 Sep 12;7:1409. doi: 10.3389/fmicb.2016.01409. eCollection 2016.
The aim of this study, was to characterize the extended-spectrum-β-lactamases (ESBLs) producing clinical strains of Escherichia coli isolated between January 2009 and June 2012 from Algerian hospitals and to determine the prevalence of 16S rRNA methylase among them. Sixty-seven ESBL-producers were detected among the 239 isolates included: 52 CTX-M-15-producers, 5 CTX-M-3-producers, 5 CTX-M-1-producers, 2 CTX-M-14-producers, 2 SHV-12-producers and one TEM-167-producer. Among the ESBL-producing strains twelve harbored 16S rRNA methylase genes: 8 rmtB and 4 armA. rmtB was located on a IncFIA plasmid and armA was located either on a IncL/M or a IncFIA plasmid. RmtB-producing isolates were genotypically related and belonged to the sequence type ST 405 whereas ArmA-producing isolates belonged to ST10, ST 167, and ST 117. This first description of 16S rRNA methylases among E. coli in Algerian hospitals pointed out the necessity to establish control measures to avoid their dissemination.
本研究的目的是对2009年1月至2012年6月间从阿尔及利亚医院分离出的产超广谱β-内酰胺酶（ESBLs）的大肠埃希菌临床菌株进行特征分析，并确定其中16S rRNA甲基化酶的流行情况。在所纳入的239株分离菌中检测到67株产ESBLs菌株：52株产CTX-M-15、5株产CTX-M-3、5株产CTX-M-1、2株产CTX-M-14、2株产SHV-12以及1株产TEM-167。在产ESBLs菌株中，有12株携带16S rRNA甲基化酶基因：8株携带rmtB，4株携带armA。rmtB位于IncFIA质粒上，armA位于IncL/M或IncFIA质粒上。产RmtB的分离菌在基因型上相关，属于序列型ST 405，而产ArmA的分离菌属于ST10、ST 167和ST 117。阿尔及利亚医院大肠埃希菌中16S rRNA甲基化酶的首次描述指出有必要建立控制措施以避免其传播。
