Tserkovsky D A, Alexandrova E N, Chalau V N, Istomin Yu P
N.N. Alexandrov National Cancer Center of Belarus, Minsk 223040, Belarus.
Exp Oncol. 2012 Dec;34(4):332-5.
The aim of this study was to investigate the low-power density sonication, sonodynamic therapy (SDT) with Photolon and combination of SDT and photodynamic therapy (PDT) with Photolon for the ablation of glioma C6 tumor model in rats.
The study was performed on 50 rats bearing glioma C6. The tumors were sonicated with/without prior intravenous injection of photosensitizer (PS) Photolon (2.5 mg/kg b.w). Sonication was performed with 0.4; 0.7 and 1.0 W/cm² power density at 1 MHz frequency for 10 min, 2.0 h after Photolon administration using BTL-5710 Sono (BTL Industries Limited, Great Britain). PDT was carried out 2.5 h after Photolon administration using diode laser with 661 nm wavelength (IMAF-AXICON, Minsk, Republic of Belarus) at doses of 50 and 100 J/cm² with 0,17 W/cm² fluence rate. Assessment of tumor response was performed by vital staining with Evans blue and pathologic examination.
The maximal tumor necrosis area that underwent sonication (1 MHz; 0.7 W/cm²; 10 min.) followed by PDT at a dose of 100 J/cm² was 100%.
This is the first report to demonstrate the benefits of sono-photodynamic therapy (SPDT) consisting of low-power density ultrasound and PDT for the treatment of malignant glioma models.
本研究的目的是研究低功率密度超声、使用Photolon的声动力疗法（SDT）以及使用Photolon的SDT与光动力疗法（PDT）联合用于大鼠胶质瘤C6肿瘤模型的消融。
对50只携带胶质瘤C6的大鼠进行研究。在静脉注射/不静脉注射光敏剂（PS）Photolon（2.5mg/kg体重）的情况下对肿瘤进行超声处理。使用BTL-5710 Sono（英国BTL Industries Limited）在1MHz频率下以0.4；0.7和1.0W/cm²的功率密度进行超声处理10分钟，在注射Photolon后2.0小时进行。在注射Photolon后2.5小时使用波长为661nm的二极管激光（白俄罗斯共和国明斯克的IMAF-AXICON）以0.17W/cm²的通量率、50和100J/cm²的剂量进行PDT。通过伊文思蓝活体染色和病理检查评估肿瘤反应。
超声处理（1MHz；0.7W/cm²；10分钟）后接着进行100J/cm²剂量的PDT，最大肿瘤坏死面积为100%。
这是第一份证明由低功率密度超声和PDT组成的声光动力疗法（SPDT）对恶性胶质瘤模型治疗有益的报告。
