Ronco G, Vineis P, Bryant M S, Skipper P L, Tannenbaum S R
Unit of Epidemiology, Local Health Administration TO I, Torino, Italy.
Br J Cancer. 1990 Apr;61(4):534-7. doi: 10.1038/bjc.1990.120.
In a previous study we found that aromatic amines, particularly 4-aminobiphenyl, formed haemoglobin adducts at higher concentrations in the blood of smokers compared to non-smokers. We re-analyse here data on haemoglobin adducts of 14 aromatic amines in order to ascertain if the inter-individual variability left unexplained by tobacco smoking could be attributed to differences in individual metabolic patterns. For this purpose we computed residuals from analysis of variance in order to adjust for individual smoking habits (type and amount of tobacco). Residuals were correlated within two clearly distinct groups: one formed by binuclear compounds (4-aminobiphenyl, 3-aminobiphenyl and 2-naphthylamine) and the other formed by all other (i.e. mononuclear) compounds. Within each group, highly statistically significant correlation coefficients were found, whereas compounds belonging to one group were not correlated to compounds in the other group. These results can be interpreted as a suggestion that two different metabolic pathways exist, one for binuclear and one for mononuclear arylamines, and that inter-individual differences in such pathways can explain part of inter-individual variability in adduct levels. This interpretation is consistent with recent animal experiments suggesting that there are different enzyme systems for the two classes of compounds.
在之前的一项研究中，我们发现，与不吸烟者相比，吸烟者血液中芳香胺，尤其是4-氨基联苯，在较高浓度下会形成血红蛋白加合物。我们在此重新分析了14种芳香胺血红蛋白加合物的数据，以确定吸烟无法解释的个体间差异是否可归因于个体代谢模式的差异。为此，我们通过方差分析计算残差，以调整个体吸烟习惯（烟草类型和数量）。残差在两个明显不同的组内具有相关性：一组由双核化合物（4-氨基联苯、3-氨基联苯和2-萘胺）组成，另一组由所有其他（即单核）化合物组成。在每组中，均发现具有高度统计学意义的相关系数，而属于一组的化合物与另一组中的化合物不相关。这些结果可以解释为，存在两种不同的代谢途径，一种用于双核芳胺，另一种用于单核芳胺，并且这些途径中的个体差异可以解释加合物水平个体间差异的一部分。这一解释与最近的动物实验一致，该实验表明这两类化合物存在不同的酶系统。
