Nie Yanfang, Liu Xiumei, Yang Xinmei, Zhao Zhongxi
School of Pharmaceutical Sciences and Center for Pharmaceutical Research & Drug Delivery Systems, Shandong University, Jinan, Shandong 250012, China.
J Chromatogr Sci. 2013 Sep;51(8):753-63. doi: 10.1093/chromsci/bms209. Epub 2013 Jan 11.
The technology of analyzing the pharmacological and toxicological properties of chiral drugs has become more commonly utilized since the knowledge of the biological actions of enantiomers has been gradually acquired during the last few decades. This work reviews the applications of chiral liquid chromatography coupled to tandem mass spectrometry (LC-MS) for the analysis of chiral pharmaceuticals since 2005. The enantioselective determinations in pharmaceutical and biomedical fields with LC-MS are classified based on three mobile phase modes, including normal phase, reversed phase and polar organic solvent, in terms of their compatibility with various ionization sources and specific applications.
在过去几十年中，随着对映体生物活性的知识逐渐积累，分析手性药物药理和毒理性质的技术得到了更广泛的应用。本文综述了自2005年以来手性液相色谱-串联质谱联用技术（LC-MS）在分析手性药物方面的应用。基于与各种电离源的兼容性和具体应用，将LC-MS在药物和生物医学领域的对映体选择性测定分为三种流动相模式，包括正相、反相和极性有机溶剂。
