Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
Department of Biology, Faculty of Science, Anadolu University, Eskisehir-Turkey.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2823-2828. doi: 10.1099/ijs.0.049551-0. Epub 2013 Jan 11.
A reddish-orange-pigmented, Gram-stain-negative, aerobic, facultatively methylotrophic strain, N4211(T), isolated from arid soil, collected from Abuja, Nigeria, was analysed by using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain N4211(T) belonged to the genus Methylobacterium. Strain N4211(T) was most closely related to Methylobacterium aquaticum GR16(T) (98.56 %), Methylobacterium platani PMB02(T) (97.95 %) and Methylobacterium variabile GR3(T) (97.2 %), and the phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97.0 %. The major ubiquinones detected were Q-10. The major fatty acids were summed feature 7 (C18 : 1 cis11/t9/t6). The DNA G+C content was 67.3 mol%. DNA-DNA relatedness of strain N4211(T) and the most closely related strains M. aquaticum DSM 16371(T) and M. platani KCTC 12901(T) were 60.0 and 48.2 %, respectively. On the basis of phenotypic, phylogenetic and DNA-DNA hybridization data, strain N4211(T) is assigned to a novel species of the genus Methylobacterium for which the name Methylobacterium tarhaniae sp. nov. is proposed. The type strain is N4211(T)( = KCTC 23615(T) = DSM 25844(T)).
一株略带红色橙色的革兰氏阴性需氧型兼性甲基营养菌 N4211(T)，从尼日利亚阿布贾的干旱土壤中分离得到，通过多相分析进行了分析。基于 16S rRNA 基因序列的系统发育分析表明，菌株 N4211(T)属于甲基杆菌属。菌株 N4211(T)与水生甲基杆菌 GR16(T)(98.56%)、木兰甲基杆菌 PMB02(T)(97.95%)和可变甲基杆菌 GR3(T)(97.2%)最为密切相关，与所有其他具有有效发表名称的甲基杆菌属物种的系统发育相似性均低于 97.0%。检测到的主要类脂醌为 Q-10。主要脂肪酸为 sum feature 7 (C18:1 cis11/t9/t6)。DNA G+C 含量为 67.3 mol%。菌株 N4211(T)与最密切相关的菌株水生甲基杆菌 DSM 16371(T)和木兰甲基杆菌 KCTC 12901(T)的 DNA-DNA 同源性分别为 60.0%和 48.2%。基于表型、系统发育和 DNA-DNA 杂交数据，菌株 N4211(T)被分配到甲基杆菌属的一个新种，命名为 Tarhaniae 甲基杆菌 sp. nov.。模式菌株为 N4211(T)(=KCTC 23615(T)=DSM 25844(T))。
