Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Department of Bio & Environmental Technology, Seoul Women's University, Seoul 01797, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2449-2456. doi: 10.1099/ijsem.0.004054. Epub 2020 Feb 25.
A gamma radiation-resistant and pink-pigmented bacterial strain, designated as 17Sr1-39, was isolated from a gamma ray-irradiated soil sample collected in the Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, flagellated, asporogenous, rod-shaped and methylotrophic. Results of 16S rRNA gene sequence analysis showed that strain 17Sr1-39 was phylogenetically related to PR1016A (97.3 %), DSM 16371 (97.2 %), PMB02 (97.0 %), IER25-16 (96.6 %), 17Sr1-28 (96.6 %) and JCM 2833 (93.4 %). The G+C content calculated based on the genome sequence was 70.4 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain 17Sr1-39 and , , , , and were 77.3-89.9 and 22-38.2 %, respectively. The predominant fatty acids were summed feature 8 (Cω7 and/or Cω6) and summed feature 3 (Cω7 and/or Cω6). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the data from phenotypic tests and genotypic differences between strain 17Sr1-39 and its close phylogenetic relatives, strain 17Sr1-39 represented a new species belonging to the genus , for which the name sp. nov. (=KACC 52905=NBRC 112874) is proposed.
一株耐γ射线并呈粉红色的细菌菌株，被命名为 17Sr1-39，从韩国采集的γ射线辐照土壤样本中分离得到。细胞革兰氏染色阴性，严格需氧，有鞭毛，无孢子，杆状，甲醇营养型。16S rRNA 基因序列分析结果表明，菌株 17Sr1-39与 PR1016A（97.3%）、DSM 16371（97.2%）、PMB02（97.0%）、IER25-16（96.6%）、17Sr1-28（96.6%）和 JCM 2833（93.4%）聚为一簇。基于基因组序列计算的 G+C 含量为 70.4mol%。菌株 17Sr1-39 与 、 、 、 、 和 的平均核苷酸同一性和 DNA-DNA 杂交值分别为 77.3-89.9%和 22-38.2%。主要脂肪酸为 sum feature 8（Cω7 和/或 Cω6）和 sum feature 3（Cω7 和/或 Cω6）。主要醌为泛醌 10，主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。基于表型测试和菌株 17Sr1-39 与其近缘种之间的基因型差异的数据，菌株 17Sr1-39 代表一个新种，属于 属，命名为 sp. nov.（=KACC 52905=NBRC 112874）。
