Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Ensuiko Sugar Refining Co., Ltd, 1-1-1 Fukuura, Kanazawa-ku, Yokohama-shi, 236-0004, Japan.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2765-2769. doi: 10.1099/ijs.0.047258-0. Epub 2013 Jan 11.
A Gram-stain-positive, non-motile, non-spore-forming, rod-shaped bacterium, designated strain K-1(T), was isolated from soil at a sucrose refinery in Japan. The strain grew at 9-37 °C (optimum, 30 °C) and at pH 6-11 (optimum, pH 7.0). Phylogenetic analysis based on the full-length 16S rRNA gene sequence of strain K-1(T) revealed that it was a member of the genus Microbacterium. High 16S rRNA gene sequence similarities were found between strains K-1(T) and both Microbacterium pumilum NBRC 101279(T) (99.7 %) and Microbacterium deminutum NRRL B-24453(T) (99.5 %). However, the DNA-DNA hybridization values between strain K-1(T) and M. pumilum NBRC 101279(T) and M. deminutum NRRL B-24453(T) were only 12 % and 10 %, respectively. The DNA G+C content of strain K-1(T) was 73 mol%. The major fatty acids of strain K-1(T) were anteiso-C15 : 0 and anteiso-C17 : 0, and the major menaquinones were MK-12 and MK-13. The diamino acid in the cell-wall peptidoglycan was lysine. On the basis of these results, strain K-1(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium saccharophilum sp. nov. is proposed. The type strain is K-1(T) (= NBRC 108778(T) = NCIMB 14782(T)).
一株革兰氏阳性、非运动、非孢子形成、杆状细菌，被命名为 K-1(T) 菌株，从日本一家蔗糖精炼厂的土壤中分离得到。该菌株在 9-37°C（最适温度为 30°C）和 pH6-11（最适 pH 值为 7.0）的条件下生长。基于 K-1(T) 菌株的全长 16S rRNA 基因序列的系统发育分析表明，它是微杆菌属的一个成员。K-1(T) 菌株与 Microbacterium pumilum NBRC 101279(T)（99.7%）和 Microbacterium deminutum NRRL B-24453(T)（99.5%）的 16S rRNA 基因序列相似度很高。然而，K-1(T) 菌株与 M. pumilum NBRC 101279(T)和 M. deminutum NRRL B-24453(T)之间的 DNA-DNA 杂交值分别只有 12%和 10%。K-1(T) 菌株的 DNA G+C 含量为 73mol%。K-1(T) 菌株的主要脂肪酸为 anteiso-C15:0 和 anteiso-C17:0，主要的menaquinones 为 MK-12 和 MK-13。细胞壁肽聚糖中的二氨基酸为赖氨酸。基于这些结果，K-1(T) 菌株被认为代表了微杆菌属的一个新种，因此建议将其命名为嗜糖微杆菌（Microbacterium saccharophilum）。该模式菌株为 K-1(T)（=NBRC 108778(T)=NCIMB 14782(T)）。
