College of Pharmacy and Life Science, Jiujiang University, Jiujiang, 332000, China.
J Antibiot (Tokyo). 2022 Dec;75(12):662-670. doi: 10.1038/s41429-022-00568-w. Epub 2022 Sep 27.
A Gram-stain positive, aerobic, rod-shaped actinobacterial strain designated as JXJ CY 27-2 was isolated from the culture of Microcystis aeruginosa FACHB-905 (Maf) collected from Lake Kunming, southwest China. The isolate was catalase positive, oxidase negative, and able to grow at 10.0-44.0 °C, pH 5.0-10.0 and 0-5.0% NaCl. Based on the 16S rRNA gene sequences, JXJ CY 27-2 showed high similarities of 98.54-98.55% with Microbacterium invictum DSM 19600, Microbacterium saccharophilum DSM 28107, and Microbacterium aoyamense DSM 19461, and less than 98.47% similarities with other members of the genus. Its major cellular fatty acids were anteiso-C and anteiso-C. The predominant menaquinones were MK-11 and MK-12. The diagnostic diamino acid in the cell wall peptidoglycan was lysine. Whole cell sugars contained mannose, ribose, galactose, rhamnose and arabinose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, and an unidentified lipid. The DNA G + C content was 69.8%. The digital DNA-DNA hybridization and average nucleotide identity values between strain JXJ CY 27-2 and its three closest similar strains were 18.4-20.3% and 74.9-75.7%, respectively. Based on the above data, strain JXJ CY 27-2 was identified as a new species of the genus Microbacterium, for which the name Microbacterium kunmingensis sp. nov. is proposed. The type strain is JXJ CY 27-2 (=CGMCC 1.17506 = KCTC 49382). Strain JXJ CY 27-2 could promote the growth of Maf by providing it with available phosphorus, nitrogen and probably other nutrients such as vitamins and indole-3-acetate.
一株革兰氏阳性、需氧、杆状放线菌菌株，命名为 JXJ CY 27-2，从中国西南部滇池采集的铜绿微囊藻 FACHB-905（Maf）的培养物中分离得到。该分离株过氧化氢酶阳性，氧化酶阴性，能够在 10.0-44.0°C、pH5.0-10.0 和 0-5.0%NaCl 的条件下生长。基于 16S rRNA 基因序列，JXJ CY 27-2 与 Microbacterium invictum DSM 19600、Microbacterium saccharophilum DSM 28107 和 Microbacterium aoyamense DSM 19461 的相似度高达 98.54-98.55%，与属内其他成员的相似度低于 98.47%。其主要细胞脂肪酸为 anteiso-C 和 anteiso-C。主要的menaquinone 为 MK-11 和 MK-12。细胞壁肽聚糖中的特征二氨基庚二酸为赖氨酸。全细胞糖含有甘露糖、核糖、半乳糖、鼠李糖和阿拉伯糖。极性脂包括双磷脂酰甘油、磷脂酰甘油、两种未鉴定的糖脂和一种未鉴定的脂。DNA G+C 含量为 69.8%。菌株 JXJ CY 27-2 与 3 株最相似菌株之间的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 18.4-20.3%和 74.9-75.7%。基于上述数据，菌株 JXJ CY 27-2 被鉴定为 Microbacterium 属的一个新种，命名为 Microbacterium kunmingensis sp. nov. 模式菌株为 JXJ CY 27-2（=CGMCC 1.17506=KCTC 49382）。菌株 JXJ CY 27-2 可以通过提供可利用的磷、氮和可能的其他营养物质（如维生素和吲哚-3-乙酸）来促进 Maf 的生长。
