State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2853-2858. doi: 10.1099/ijs.0.049304-0. Epub 2013 Jan 11.
A yellow, rod-shaped, Gram-negative, facultatively aerobic, gliding bacterium, designed strain P7-3-5(T), was isolated from intertidal sand of the Yellow Sea, China. Analysis of 16S rRNA gene sequences revealed that strain P7-3-5(T) formed a distinct lineage within the family Flavobacteriaceae, sharing 94.2-96.9 % sequence similarity with type strains of species of the most closely related genera, including Hyunsoonleella, Jejuia, Marinivirga and Algibacter. The strain grew at 4-40 °C and with 0.5-5.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed gelatin and DNA. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 and the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine (PE), three unidentified aminolipids (AL1-3) and four unidentified lipids (L1-4). The genomic DNA G+C content of strain P7-3-5(T) was 32.1 mol%. Data from this polyphasic study suggest that strain P7-3-5(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Arenitalea lutea gen. nov., sp. nov. is proposed. The type strain of Arenitalea lutea is P7-3-5(T) ( = CGMCC 1.12213(T) = KACC 16457(T)).
一株黄色、杆状、革兰氏阴性、兼性需氧、滑行的细菌，设计菌株 P7-3-5(T)，从中国黄海的潮间带沙中分离得到。16S rRNA 基因序列分析表明，菌株 P7-3-5(T)在黄杆菌科内形成一个独特的谱系，与最密切相关属的模式菌株的序列相似性为 94.2-96.9%，包括 Hyunsoonleella、Jejuia、Marinivirga 和 Algibacter。该菌株在 4-40°C 和 0.5-5.0%（w/v）NaCl 下生长。它将硝酸盐还原为亚硝酸盐，并水解明胶和 DNA。主要细胞脂肪酸为 iso-C15:0、iso-C15:1 G 和 anteiso-C15:0，主要呼吸醌为 MK-6。极性脂包括磷脂酰乙醇胺（PE）、三种未鉴定的胺脂（AL1-3）和四种未鉴定的脂（L1-4）。菌株 P7-3-5(T)的基因组 DNA G+C 含量为 32.1mol%。多相研究的数据表明，菌株 P7-3-5(T)代表黄杆菌科一个新属中的一个新种，建议将其命名为Arenitalea lutea 属，新种。Arenitalea lutea 的模式菌株为 P7-3-5(T)（=CGMCC 1.12213(T)=KACC 16457(T)）。
