Soares T N, Sant'ana L L, de Oliveira L K, Telles M P C, Collevatti R G
Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil
Genet Mol Res. 2013 Jan 4;12(3):3146-9. doi: 10.4238/2013.January.4.24.
Microsatellite markers were transferred from the cashew, Anarcadium occidentale, to Anacardium humile (Anacardiaceae), a Neotropical shrub from the Brazilian savanna, that produces an edible nut and pseudo-fruit. We tested 14 microsatellite primers from A. occidentale on A. humile. Polymorphism of each microsatellite locus was analyzed based on 58 individuals from three populations. Twelve loci amplified successfully and presented 2 to 9 alleles; expected heterozygosity ranged from 0.056 to 0.869. These 12 microsatellite loci provide a new tool for the generation of fundamental population genetic data for devising conservation strategies for A. humile.
微卫星标记已从腰果（Anarcadium occidentale）转移至巴西坚果（Anacardium humile，漆树科），后者是一种来自巴西稀树草原的新热带灌木，能产出可食用的坚果和假果。我们在巴西坚果上测试了来自腰果的14个微卫星引物。基于来自三个种群的58个个体，分析了每个微卫星位点的多态性。12个位点成功扩增，并呈现出2至9个等位基因；预期杂合度范围为0.056至0.869。这12个微卫星位点为生成巴西坚果基础种群遗传数据以制定保护策略提供了新工具。
