Hoerter James D, Bradley Patrick, Casillas Alexandria, Chambers Danielle, Weiswasser Brandon, Clements Lauren, Gilbert Sarah, Jiao Albert
Department of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
J Skin Cancer. 2012;2012:571087. doi: 10.1155/2012/571087. Epub 2012 Dec 18.
What is the cellular origin of melanoma? What role do melanocyte stem cells (MSC) and other melanocyte precursors play in the development of melanoma? Are MSCs and other latent melanocyte precursors more susceptible to solar radiation? These and many other questions can be very effectively addressed using the zebrafish model. Zebrafish have a robust regenerative capability, permitting the study of how MSCs are regulated and recruited at specific times and places to generate the pigment pattern following fin amputation or melanocyte ablation. They can be used to determine the effects of environmental radiation on the proliferation, survival, repair, and differentiation of MSCs. Our lab is using zebrafish to investigate how UVA- (320-400 nm) and UVB- (290-320 nm) induced damage to MSCs may contribute to the development of melanoma. A review is given of MSCs in zebrafish as well as experimental techniques and drugs for manipulating MSC populations. These techniques can be used to design experiments to help answer many questions regarding the role of MSCs or melanocyte precursors in the formation of melanoma stem cells and tumors following exposure to UVA/UVB radiation.
黑色素瘤的细胞起源是什么？黑素细胞干细胞（MSC）和其他黑素细胞前体在黑色素瘤的发展中起什么作用？MSC和其他潜在的黑素细胞前体对太阳辐射更敏感吗？使用斑马鱼模型可以非常有效地解决这些以及许多其他问题。斑马鱼具有强大的再生能力，这使得研究在鳍截肢或黑素细胞消融后，MSC如何在特定时间和地点被调节和募集以产生色素模式成为可能。它们可用于确定环境辐射对MSC增殖、存活、修复和分化的影响。我们实验室正在使用斑马鱼来研究UVA（320 - 400nm）和UVB（290 - 320nm）诱导的对MSC的损伤如何可能导致黑色素瘤的发展。本文综述了斑马鱼中的MSC以及用于操纵MSC群体的实验技术和药物。这些技术可用于设计实验，以帮助回答许多关于MSC或黑素细胞前体在暴露于UVA/UVB辐射后在黑色素瘤干细胞和肿瘤形成中的作用的问题。
