Jana Debarshi, Das Soumen, Sarkar Diptendra Kumar, Mandal Syamsundar, Maji Abhiram, Mukhopadhyay Madhumita
Comprehensive Breast Service and Breast Cancer Research Unit, Breast Service, Institute of Post Graduate Medical Education and Research (IPGME and R) and Seth Sukhlal Karnani Memorial Hospital (SSKM) Kolkata, India.
Asian Pac J Cancer Prev. 2012;13(11):5511-5. doi: 10.7314/apjcp.2012.13.11.5511.
The nuclear factor κB (NF-κB) is a super family of transcription factors which plays important roles in development and progression of cancer. The present investigation concerns NF-κB /p65 activity in human breast cancers with overexpression of ER, PR, HER-2/neu, as well as the significance of p65 expression with regard to menopausal status, stage, grade, tumor size, nodal status, and NPI of invasive ductal carcinomas in Eastern India.
In this hospital based study 57 breast cancer patients attending a Breast Clinic of a reputed institute of Eastern India were assessed for p65 protein expression in breast tumor tissue samples by Western blotting. ER, PR and HER-2/neu expression was determined by immunohistochemistry.
NF-κB/p65 was significantly associated with advanced stage, large tumor size (≥5 cm), high grade, negative ER, negative PR, and positive HER-2/neu. High NF-κB/p65 expression was more frequent in patients with a high NPI (NPI≥5.4, 84.6%) compared with low NPI (<5.4, 44.4%) and this association was statistically significant (p=0.002).
NF-κB/p65 overexpression was associated with advanced stage, large tumor size, high grade, and high NPI which are poor prognostic factors linked to enhanced aggressiveness of the disease. NF-κB/p65 expression implies aggressive biological behavior of breast cancer and this study validates significant association of NF-κB /p65 overexpression with negative estrogen and progesterone receptor status and overexpression of HER-2/neu oncoprotein. In our good clinical practice, patients with NF-κB positive tumors need to be treated aggressively.
核因子κB（NF-κB）是一类转录因子超家族，在癌症的发生和发展中发挥重要作用。本研究关注雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2/神经生长因子受体（HER-2/neu）过表达的人类乳腺癌中NF-κB/p65的活性，以及在印度东部浸润性导管癌中，p65表达与绝经状态、分期、分级、肿瘤大小、淋巴结状态和诺丁汉预后指数（NPI）的关系。
在这项基于医院的研究中，对印度东部一家著名机构乳腺门诊的57例乳腺癌患者，通过蛋白质免疫印迹法评估乳腺肿瘤组织样本中p65蛋白的表达。通过免疫组织化学法测定ER、PR和HER-2/neu的表达。
NF-κB/p65与晚期、肿瘤大（≥5 cm）、高级别、ER阴性、PR阴性和HER-2/neu阳性显著相关。与低NPI（<5.4，44.4%）患者相比，高NPI（NPI≥5.4，84.6%）患者中NF-κB/p65高表达更为常见，且这种相关性具有统计学意义（p = 0.002）。
NF-κB/p65过表达与晚期、肿瘤大、高级别和高NPI相关，这些都是与疾病侵袭性增强相关的不良预后因素。NF-κB/p65表达意味着乳腺癌具有侵袭性生物学行为，本研究证实了NF-κB/p65过表达与雌激素和孕激素受体阴性状态以及HER-2/neu癌蛋白过表达之间的显著相关性。在我们的良好临床实践中，NF-κB阳性肿瘤患者需要积极治疗。
