Department of Agricultural Food and Nutritional Science, Faculty of Agricultural Life and Environmental Sciences, University of Alberta , Edmonton, Alberta, Canada.
J Agric Food Chem. 2013 Mar 6;61(9):2120-9. doi: 10.1021/jf3046076. Epub 2013 Feb 20.
Egg protein ovotransferrin derived peptides (IRW and IQW) can attenuate tumor necrosis factor (TNF) induced inflammatory responses and oxidative stress in endothelial cells. The present study investigates the structural requirements and molecular mechanisms underlying these events. Whereas IRW significantly inhibited TNF-induced up-regulation of intercellular cell adhesion molecule-I (ICAM-1) and vascular cell adhesion molecule-I (VCAM-1), IQW could inhibit only the up-regulation of ICAM-1. The anti-inflammatory effects of these peptides appeared to be mediated by the nuclear factor-κB (NF-κB) pathway, which was differentially regulated by IRW and IQW. Both IRW and IQW exhibited antioxidant effects as shown by reduction of TNF-induced superoxide generation. The structural integrity of these peptides was essential for their activities, because dipeptides or the combination of constituent amino acids did not exhibit the same effect. This study demonstrated the significance of the structural integrity of these two tripeptides in attenuating endothelial inflammation and oxidative stress, indicating their potential as nutraceuticals.
卵转铁蛋白衍生肽（IRW 和 IQW）可减轻肿瘤坏死因子（TNF）诱导的内皮细胞炎症反应和氧化应激。本研究探讨了这些事件的结构要求和分子机制。IRW 可显著抑制 TNF 诱导的细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的上调，而 IQW 仅能抑制 ICAM-1 的上调。这些肽的抗炎作用似乎是通过核因子-κB（NF-κB）途径介导的，IRW 和 IQW 对该途径的调节存在差异。IRW 和 IQW 均表现出抗氧化作用，可减少 TNF 诱导的超氧生成。这些肽的结构完整性对于其活性至关重要，因为二肽或组成氨基酸的组合没有表现出相同的效果。本研究表明这两个三肽在减轻内皮炎症和氧化应激中的结构完整性的重要性，表明它们具有作为营养保健品的潜力。
