Tazaki Masakazu, Endoh Takayuki, Kobayashi Hiroshi, Nobushima Hiromi, Shibukawa Yoshiyuki, Tsumura Maki, Sato Masaki, Ubaidus Sobhan, Sueishi Kenji
Department of Physiology, Tokyo Dental College, Chiba, Japan.
Bull Tokyo Dent Coll. 2012;53(4):203-6. doi: 10.2209/tdcpublication.53.203.
Osteoblasts play a major role in bone formation. Osteoblasts employ intracellular Ca(2+) as a second messenger to modulate hormonal responses and a cofactor for bone mineralization. Adrenomedullin (ADM) promotes osteoblast growth and proliferation, inducing an increase in bone mass. Voltage-dependent Ca(2+) channels (VDCCs) mediate the influx of Ca(2+) in response to membrane depolarization. Voltage-dependent Ca(2+) channels serve as crucial mediators of many Ca(2+)-dependent functions, including growth of bone and regulation of proliferation. The purpose of this study was to investigate the effects of ADM on VDCC currents in osteoblasts using a patch-clamp recording method. To our knowledge, the data presented here demonstrate for the first time that ADM facilitates VDCCs in osteoblasts.
成骨细胞在骨形成过程中发挥着主要作用。成骨细胞利用细胞内钙离子（Ca²⁺）作为第二信使来调节激素反应，并作为骨矿化的辅助因子。肾上腺髓质素（ADM）促进成骨细胞的生长和增殖，导致骨量增加。电压依赖性钙离子通道（VDCCs）介导钙离子在膜去极化时的内流。电压依赖性钙离子通道是许多钙离子依赖性功能的关键介质，包括骨骼生长和增殖调节。本研究的目的是使用膜片钳记录方法研究ADM对成骨细胞中VDCC电流的影响。据我们所知，此处呈现的数据首次证明ADM可促进成骨细胞中的VDCCs。
