Instituto de la Grasa, Consejo Superior de Investigaciones Cientı́ficas (CSIC) , Avenida Padre Garcı́a Tejero 4, Sevilla 41012, Spain.
J Agric Food Chem. 2013 Feb 13;61(6):1235-48. doi: 10.1021/jf303772p. Epub 2013 Feb 1.
A liquid phase rich in bioactive compounds, such as phenols and sugars, is obtained from olive oil waste by novel thermal treatment. Two groups of fractions with common characteristics were obtained and studied after thermal treatment, acid hydrolysis, and separation by ultrafiltration, chromatography, and finally Superdex Peptide HR. In the first group, which eluted at the same time as oligosaccharides with a low DP (4-2), an oleosidic secoiridoid structure conjugated to a phenolic compound (hydroxytyrosol) was identified as oleuropeinic acid, and three possible structures were detected. In the second group, glucosyl structures formed by hydroxytyrosol and one, two, or three units of glucose or by tyrosol and glucose have been proposed. Verbascoside, a heterosidic ester of caffeic acid, in which hydroxytyrosol is linked to rhamnose-glucose or one of its isomers was also identified. Neutral oligosaccharides bound to a phenol-containing compound could be antioxidant-soluble fibers with bioactive properties.
新型热疗从橄榄油废料中提取富含生物活性化合物（如酚类化合物和糖）的液相。对热疗、酸水解以及超滤、色谱和最后 Superdex Peptide HR 分离后的两组具有共同特征的馏分进行了研究。在第一组中，与低 DP（4-2）的低聚糖同时洗脱的物质被鉴定为与酚类化合物（羟基酪醇）结合的橄榄苦苷型裂环环烯醚萜，检测到三种可能的结构。在第二组中，提出了由羟基酪醇和一个、两个或三个葡萄糖单元或由酪醇和葡萄糖形成的葡萄糖基结构。还鉴定了咖啡酸杂糖苷委陵菜糖苷，其中羟基酪醇与鼠李糖葡萄糖或其异构体之一相连。与含酚化合物结合的中性低聚糖可能是具有生物活性的抗氧化可溶性纤维。
