Li Hui, Yao Weifeng, Liu Qinan, Xu Jia, Bao Beihua, Shan Mingqiu, Cao Yudan, Cheng Fangfang, Ding Anwei, Zhang Li
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
Molecules. 2017 Apr 26;22(5):689. doi: 10.3390/molecules22050689.
Ligustri Lucidi Fructus (LLF), the fruit of Ait. (Oleaceae), has been used as a common herbal medicine in clinical practice in China for nearly 2000 years. In most cases, LLF is prescribed in decoctions in the form of processed products rather than crude drugs. In this study, an ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-ESI-Q-TOF-MS) method was established for rapid separation and identification of multiple constituents in the 80% methanol extract of processed-LLF. A total of 50 compounds (one phenylethanoid, seven phenylethanoid glycosides, seven flavonoids, 25 iridoids, nine triterpenoids and one cyclohexanecarboxylic acid) were either unambiguously identified or tentatively characterized with the aid of authentic standards or published data. Luteolin-7--rutinoside, oleoside and secologanoside were detected in LLF for the first time. This study enriches the chemical profiling of processed-LLF and could provide valuable information for the quality control and further investigation of processed-LLF and crude LLF.
女贞子，为木犀科植物女贞Ligustrum lucidum Ait.的果实，在中国临床实践中作为常用草药已使用了近2000年。在大多数情况下，女贞子是以炮制后的产品形式而非生药形式入药汤剂。本研究建立了一种超高效液相色谱-电喷雾电离-四极杆-飞行时间质谱联用（UHPLC-ESI-Q-TOF-MS）方法，用于快速分离和鉴定炮制女贞子80%甲醇提取物中的多种成分。借助标准品或已发表数据，共明确鉴定或初步表征了50种化合物（1种苯乙醇类、7种苯乙醇苷类、7种黄酮类、25种环烯醚萜类、9种三萜类和1种环己烷羧酸）。首次在女贞子中检测到木犀草素-7-O-芸香糖苷、橄榄苦苷和裂环马钱子苷。本研究丰富了炮制女贞子的化学图谱，可为炮制女贞子及生女贞子的质量控制和进一步研究提供有价值的信息。
