Cinco M, De Giovannini R
Istituto di Microbiologia, University of Trieste, Italy.
J Clin Microbiol. 1993 Feb;31(2):440-3. doi: 10.1128/jcm.31.2.440-443.1993.
Four Borrelia burgdorferi strains isolated in the same restricted geographic area share different protein patterns on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The use of polyclonal rabbit antisera and a battery of monoclonal antibodies directed toward the immunodominant proteins OspA, OspB, and pC also revealed different epitope distributions and specificities of these antigens on the strains examined. For the first time, a computerized analysis of these phenotypic characters was done mainly by cluster analysis. The computerized analysis revealed the levels of similarities among the strains and indicated, on a quantitative basis, that one of them is much closer to the American strain B31 than to the other strains.
在同一限定地理区域分离出的四株伯氏疏螺旋体菌株，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳上呈现出不同的蛋白质图谱。使用兔多克隆抗血清以及一系列针对免疫显性蛋白OspA、OspB和pC的单克隆抗体，也揭示了这些抗原在受试菌株上不同的表位分布和特异性。首次主要通过聚类分析对这些表型特征进行了计算机分析。计算机分析揭示了菌株之间的相似程度，并在定量基础上表明，其中一株与美国菌株B31的亲缘关系比与其他菌株更近。
