猪群中对胸膜肺炎放线杆菌104千道尔顿溶血素血清反应阳性的流行情况。
Prevalence of seroreactors to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae in swine herds.
作者信息
Devenish J, Rosendal S, Bossé J T, Wilkie B N, Johnson R
机构信息
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
出版信息
J Clin Microbiol. 1990 Apr;28(4):789-91. doi: 10.1128/jcm.28.4.789-791.1990.
A rabbit homologous polyclonal antiserum to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was specifically produced and used in an antigen capture enzyme-linked immunosorbent assay (ELISA) to detect swine serum antibodies to this potentially important virulence factor. Sera from pigs experimentally infected with the most common disease-producing serotypes (serotypes 1, 2, 5, and 7) of A. pleuropneumoniae produced positive results in this ELISA. Of 144 serum samples collected from 10 herds free of pleuropneumonia and 155 serum samples from 11 herds with a history of the disease, 68 (47%) and 148 (95%), respectively, were found positive by the ELISA. In addition, pigs naturally infected with Actinobacillus suis produced antibodies which seroreacted in this ELISA. The results indicated that a high proportion of swine have antibodies seroreactive with the 104-kilodalton hemolysin produced by A. pleuropneumoniae.
特异性制备了针对胸膜肺炎放线杆菌1型菌株CM - 5的104千道尔顿溶血素的兔同源多克隆抗血清，并将其用于抗原捕获酶联免疫吸附测定（ELISA），以检测猪血清中针对这种潜在重要毒力因子的抗体。在该ELISA中，来自经胸膜肺炎放线杆菌最常见致病血清型（血清型1、2、5和7）实验感染的猪的血清产生了阳性结果。从10个无胸膜肺炎的猪群收集的144份血清样本和11个有该病病史的猪群的155份血清样本中，分别有68份（47%）和148份（95%）通过ELISA检测为阳性。此外，自然感染猪放线杆菌的猪产生的抗体在该ELISA中发生血清反应。结果表明，很大比例的猪具有与胸膜肺炎放线杆菌产生的104千道尔顿溶血素发生血清反应的抗体。
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