用于检测胸膜肺炎放线杆菌2型抗体的阻断酶联免疫吸附测定
Blocking enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2.
作者信息
Nielsen R, Plambeck T, Foged N T
机构信息
National Veterinary Laboratory, Copenhagen, Denmark.
出版信息
J Clin Microbiol. 1991 Apr;29(4):794-7. doi: 10.1128/jcm.29.4.794-797.1991.
A blocking enzyme-linked immunosorbent assay (ELISA), based upon a polyclonal rabbit antiserum specific to Actinobacillus pleuropneumoniae serotype 2, was developed for the detection of antibodies to A. pleuropneumoniae serotype 2 in pigs. By testing sera from pigs experimentally infected with the 11 recognized serotypes of A. pleuropneumoniae, the assay was proven to be specific for A. pleuropneumoniae serotype 2. With field sera from herds infected with A. pleuropneumoniae serotype 2, the assay was found to be more sensitive than the complement fixation test. Positive results were not observed with field sera from herds known to be free from Actinobacillus infection or with sera from two herds infected with either A. pleuropneumoniae serotype 6 or 8. The high diagnostic sensitivity and specificity of the blocking ELISA will make it useful in field diagnostic work.
基于对胸膜肺炎放线杆菌2型具有特异性的兔多克隆抗血清，开发了一种阻断酶联免疫吸附测定（ELISA）法，用于检测猪血清中针对胸膜肺炎放线杆菌2型的抗体。通过检测经胸膜肺炎放线杆菌11种公认血清型实验感染的猪的血清，该测定法被证明对胸膜肺炎放线杆菌2型具有特异性。对于感染胸膜肺炎放线杆菌2型的猪群的现场血清，该测定法比补体结合试验更敏感。在已知无放线杆菌感染的猪群的现场血清或感染胸膜肺炎放线杆菌6型或8型的两个猪群的血清中未观察到阳性结果。阻断ELISA的高诊断敏感性和特异性将使其在现场诊断工作中有用。
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