School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Carbohydr Polym. 2017 May 1;163:10-19. doi: 10.1016/j.carbpol.2017.01.021. Epub 2017 Jan 6.
Three-phase partitioning (TPP), which is a simple, efficient, and green bioseparation technique, was used to extract and separate polysaccharides from Corbicula fluminea (CFPS). The optimal parameters with a high yield of 9.32% were as follows: mass fraction of (NH)SO, 20.0% (w/v); amount of t-butanol, 9.8mL; temperature, 35.3°C; extraction time, 30min; and pH 6.0. The purified CFPS after TPP consisted of d-glucose, d-glucosamine, and d-mannose in a molar ratio of 57.1: 5.6:1.0 with high purity (86.5%) and different molecular weights (1311.1 and 41.5-92.8kDa). Amino acid analysis, UV-vis absorption and Fourier transform-infrared spectra indicated that the purified CFPS was a proteoglycan with O-glycosidic bonds. Moreover, the purified CFPS possessed strong free-radical scavenging abilities and antioxidant activities in vitro. The obtained Trolox equivalent antioxidant capacity and ferric-reducing ability of plasma values were 95.01μmol Trolox/g sample and 38.30μmol Fe/g sample, respectively.
三相分配（TPP）是一种简单、高效、绿色的生物分离技术，用于从河蚬（CFPS）中提取和分离多糖。最佳参数为：（NH）SO 质量分数为 20.0%（w/v）；叔丁醇用量为 9.8mL；温度为 35.3°C；提取时间为 30min；pH 值为 6.0。TPP 后得到的纯化 CFPS 由摩尔比为 57.1:5.6:1.0 的 d-葡萄糖、d-葡萄糖胺和 d-甘露糖组成，具有高纯度（86.5%）和不同的分子量（1311.1 和 41.5-92.8kDa）。氨基酸分析、紫外可见吸收和傅里叶变换红外光谱表明，纯化的 CFPS 是一种带有 O-糖苷键的蛋白聚糖。此外，纯化的 CFPS 在体外具有较强的自由基清除能力和抗氧化活性。获得的 Trolox 当量抗氧化能力和血浆还原能力值分别为 95.01μmol Trolox/g 样品和 38.30μmol Fe/g 样品。
