Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
Carbohydr Polym. 2013 Feb 15;92(2):2212-7. doi: 10.1016/j.carbpol.2012.12.013. Epub 2012 Dec 13.
Two acidic polysaccharides (MDP-A1 and MDP-A2) were isolated from the rhizome of Menispermum dauricum and their apparent molecular weight are 9.1×10(4) and 5.8×10(4) Da, respectively. Both contained glucose, mannose, galactose, arabinose, glucuronic acid and galacturonic acid, but differed in the molar ratio. We also investigated the antitumor activities and mechanisms of MDP-A1 and MDP-A2 in human ovarian carcinoma SKOV3 cells. The MTT assay showed that MDP-A1 and MDP-A2 were able to inhibit cell proliferation of SKOV3 cells in a bell-shaped concentration-response manner, due to a significant increase in the number of apoptotic cells. Furthermore, treatment with MDP-A1 or MDP-A2 caused significant induction of caspase-3 and caspase-8, but slightly affected caspase-9 activity. In addition, a significant reduction in tumor volume was observed in mice treated with MDP-A1 or MDP-A2. Taken together, these results suggested that MDP-A1 and MDP-A2 had a potential application as natural antitumor drugs.
从防己科蝙蝠葛属植物蝙蝠葛中分离得到两种酸性多糖（MDP-A1 和 MDP-A2），其相对分子质量分别为 9.1×10(4)和 5.8×10(4)。两种多糖均由葡萄糖、甘露糖、半乳糖、阿拉伯糖、葡萄糖醛酸和半乳糖醛酸组成，但摩尔比不同。还研究了 MDP-A1 和 MDP-A2 对人卵巢癌细胞 SKOV3 的抗肿瘤活性及其机制。MTT 法结果表明，MDP-A1 和 MDP-A2 对 SKOV3 细胞的增殖具有明显的抑制作用，呈钟形浓度-反应关系，这是由于凋亡细胞数量的显著增加。此外，用 MDP-A1 或 MDP-A2 处理会导致 caspase-3 和 caspase-8 的显著诱导，但对 caspase-9 活性的影响较小。此外，在 MDP-A1 或 MDP-A2 处理的小鼠中观察到肿瘤体积显著减小。综上所述，这些结果表明 MDP-A1 和 MDP-A2 具有作为天然抗肿瘤药物的应用潜力。
