Campos Edson Alves, Correr Gisele Maria, Leonardi Denise Piotto, Barato-Filho Flares, Gonzaga Carla Castiglia, Zielak João César
Master of Science Program, Positivo University, Brazil.
J Dent. 2009 Feb;37(2):108-14. doi: 10.1016/j.jdent.2008.10.003. Epub 2008 Nov 20.
The purpose of this study was to investigate the effects of chlorhexidine (CHX) digluconate at 0.2% and 2% on dentin bonding durability of etch-and-rinse and self-etch adhesive systems.
In this study were used 24 extracted non-carious human third-molars. The occlusal surfaces of the molar crowns were removed with a low-speed diamond saw to expose flat dentin surfaces. The tested materials were Single-Bond (SB) (two-step etch-and-rinse adhesive) and Clearfil Tri S Bond (CTSB) (all-in-one self-etch adhesive) used in association or not with CHX at 0.2% and 2%. The bonding systems were applied according to manufacturer's instructions and followed by composite application (Z250). For each condition, half of the specimens was immediately submitted to microtensile test and half of them was submitted to long-term storage of 6 months under simulated pulpal pressure and thermo-mechanical stressing before testing. The data were analyzed using Two-Way ANOVA and Tukey post hoc test (alpha=0.05). Failure patterns of the specimens were observed using scanning electron microscopy.
The falling % in bond strength over the 6-month period was: SB control-43.64%; SB/0.2%CHX-23.79%; SB/2%CHX-26.42%; CTSB control-40.94%; CTSB/0.2%CHX-37.07%; CTSB/2%CHX-22.14%. The fracture modes were predominantly adhesive, mainly in the specimens of terminal groups.
CXH digluconate at 2% was able to diminish loss of microtensile bond strength over time associated to both etch-and-rinse and self-etch adhesives. Lower concentration of CHX (0.2%) was not able to diminish the loss of bond strength over time when associated to the self-etch adhesive CTSB.
本研究旨在调查0.2%和2%的葡萄糖酸氯己定（CHX）对酸蚀冲洗和自酸蚀粘结系统牙本质粘结耐久性的影响。
本研究使用了24颗拔除的无龋人类第三磨牙。用低速金刚石锯去除磨牙牙冠的咬合面，以暴露平坦的牙本质表面。测试材料为单键（SB）（两步酸蚀冲洗粘结剂）和Clearfil Tri S Bond（CTSB）（一体化自酸蚀粘结剂），分别单独使用或与0.2%和2%的CHX联合使用。按照制造商的说明应用粘结系统，随后应用复合树脂（Z250）。对于每种情况，一半的标本立即进行微拉伸测试，另一半在模拟牙髓压力和热机械应力下长期储存6个月后再进行测试。使用双向方差分析和Tukey事后检验（α=0.05）分析数据。使用扫描电子显微镜观察标本的断裂模式。
6个月期间粘结强度下降的百分比为：SB对照组-43.64%；SB/0.2%CHX-23.79%；SB/2%CHX-26.42%；CTSB对照组-40.94%；CTSB/0.2%CHX-37.07%；CTSB/2%CHX-22.14%。断裂模式主要为粘结性，主要发生在终末组的标本中。
2%的葡萄糖酸氯己定能够减少与酸蚀冲洗和自酸蚀粘结剂相关的微拉伸粘结强度随时间的损失。当与自酸蚀粘结剂CTSB联合使用时，较低浓度的CHX（0.2%）不能减少粘结强度随时间的损失。
