Saito Akemi, Tsurikisawa Naomi, Oshikata Chiyako, Nakazawa Takuya, Yasueda Hiroshi, Akiyama Kazuo
Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Sagamihara, Japan.
Arerugi. 2012 Nov;61(11):1657-64.
Several allergen sampling methods are available for the assessment of personal or indirect exposure to indoor allergens. As an index of exposure to inhalant allergens, assays of the amount of airborne allergens directly reflect personal exposure.
We evaluated the Petri dish sampling method of assessing the level of airborne Dermatophagoides dust mite group 1 (Der 1) allergens.
We collected settling dust samples from one person's bedroom over a period of 2 years by using a Petri dish, adhesive tape, and a vacuumed reservoir. We also collected settling dust samples from the bedrooms of 42 asthma patients by using a Petri dish and adhesive tape. The amounts of Der 1 collected on the Petri dishes and adhesive tapes were measured by sensitive fluorometric ELISA.
Der 1 was detected in all samples by using a Petri dish. The mean coefficient of variation was approximately 15%. We found that Petri dishes set at lower sampling heights contained more Der 1 than those higher up. There were also seasonal changes in the amounts of Der 1 collected, with the highest amounts collected from summer to autumn, and the lowest amounts collected in winter.
The Petri dish sampling method for collecting settling Der 1 is very simple and can be used as an alternative to personal air sampling, especially in large-scale studies.
有几种过敏原采样方法可用于评估个人对室内过敏原的直接或间接暴露情况。作为吸入性过敏原暴露的指标，空气中过敏原含量的检测可直接反映个人暴露情况。
我们评估了用于评估空气中粉尘螨1组（Der 1）过敏原水平的培养皿采样方法。
我们使用培养皿、胶带和真空收集器在两年时间内从一个人的卧室收集沉降灰尘样本。我们还使用培养皿和胶带从42名哮喘患者的卧室收集沉降灰尘样本。通过灵敏的荧光酶联免疫吸附测定法测量培养皿和胶带上收集到的Der 1的量。
使用培养皿在所有样本中均检测到Der 1。平均变异系数约为15%。我们发现，采样高度较低的培养皿中所含Der 1比采样高度较高的培养皿更多。收集到的Der 1的量也存在季节性变化，夏季至秋季收集量最高，冬季收集量最低。
用于收集沉降Der 1的培养皿采样方法非常简单，可作为个人空气采样的替代方法，尤其是在大规模研究中。
