Department of Ophthalmology, Ankara Training and Research Hospital, Ankara, Turkey.
Ophthalmic Res. 2013;49(4):199-204. doi: 10.1159/000345451. Epub 2013 Jan 10.
The aim of the present study was to evaluate the cicatricial repair of a corneal artificial perforation in rats with 10-0 nylon suture, N-butyl-2-cyanoacrylate (NBCA) adhesive, or NBCA + methacryloxysulfolane (NBCA-MS) adhesive through microscopic and histological assays.
Twenty Wistar rats were randomly divided into 4 groups each containing 5 rats: (1) control group (corneal trauma without suturing and tissue adhesives), (2) suture group, (3) NBCA group and (4) NBCA-MS group. A central full-thickness 2-mm laceration was performed in the left eyes of the studied rats in all 4 groups. The presence of corneal edema, corneal neovascularization and tissue adhesive/suture were evaluated. On the 21st day, the rats were sacrificed and histological examination was performed to determine irregularity of corneal layers, superficial epithelization, polymorphonuclear leucocytes and neovascularization.
Tissue adhesives were as effective as suturing in closing full-thickness corneal wounds and no difference in postoperative healing was observed clinically. As for the histological results, suture-treated eyes had persistent corneal irregularity that can limit visual acuity and may also lead to astigmatism.
The use of tissue adhesives constitutes a viable alternative clinical procedure to conventional sutures. Possible influences on astigmatism are hypothetical, as no objective measure of astigmatism was performed in the test animals.
本研究旨在通过显微镜和组织学检查评估 10-0 尼龙缝线、N-丁基-2-氰基丙烯酸酯（NBCA）胶粘剂或 NBCA+甲基丙烯酰氧基磺烷（NBCA-MS）胶粘剂修复大鼠角膜人工穿孔的瘢痕修复效果。
将 20 只 Wistar 大鼠随机分为 4 组，每组 5 只：（1）对照组（角膜创伤未缝合和使用组织胶粘剂），（2）缝线组，（3）NBCA 组和（4）NBCA-MS 组。在所有 4 组大鼠的左眼均进行中央全层 2mm 撕裂伤。评估角膜水肿、角膜新生血管形成和组织胶粘剂/缝线的存在情况。在第 21 天，处死大鼠并进行组织学检查，以确定角膜各层的不规则性、浅表上皮化、多形核白细胞和新生血管形成。
组织胶粘剂在闭合全层角膜伤口方面与缝线同样有效，临床观察到术后愈合无差异。就组织学结果而言，缝线处理的眼睛角膜不规则持续存在，这可能会限制视力，也可能导致散光。
使用组织胶粘剂是一种可行的替代传统缝线的临床方法。对散光的可能影响是假设的，因为在实验动物中未进行散光的客观测量。
