Kholodenko I V, Yarygin K N, Gubsky L V, Konieva A A, Tairova R T, Povarova O V, Kholodenko R V, Burunova V V, Yarygin V N, Skvortsova V I
V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2012 Nov;154(1):118-23. doi: 10.1007/s10517-012-1890-6.
Mesenchymal stem cells from human placenta obtained after term natural delivery were cultured and labeled with vital dye Dil of magnetic fluorescing microparticles. The labeled cells were transplanted intravenously to rats with occlusion of the median cerebral artery. Penetration of cells through the brain-blood barrier and their distribution in the brain of experimental animals were studied on serial cryostat sections. Two models of cerebral artery occlusion associated with different traumatic consequences were used. The efficiency of crossing the blood-brain barrier by transplanted cells, the number of mesenchymal cells attaining the ischemic focus and neurogenic zones, and the time of death of transplanted cells largely depended on the degree and nature of injury to the central nervous system, which should be taken into account when planning the experiments for evaluation of the effects of cell therapy on the models of neurological diseases and in clinical studies in the field of regenerative neurology.
足月自然分娩后获取的人胎盘间充质干细胞进行培养，并用磁性荧光微粒的活性染料Dil进行标记。将标记后的细胞静脉注射到大脑中动脉闭塞的大鼠体内。在连续的低温恒冷切片上研究细胞穿过血脑屏障的情况及其在实验动物大脑中的分布。使用了两种与不同创伤后果相关的脑动脉闭塞模型。移植细胞穿过血脑屏障的效率、到达缺血灶和神经发生区的间充质细胞数量以及移植细胞的死亡时间在很大程度上取决于中枢神经系统损伤的程度和性质，在规划评估细胞疗法对神经疾病模型的影响的实验以及再生神经学领域的临床研究时应考虑到这一点。
