3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho , Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
Biomacromolecules. 2013 Mar 11;14(3):743-51. doi: 10.1021/bm301833z. Epub 2013 Feb 6.
Liquified capsules featuring (i) an external shell by layer-by-layer assembly of poly(l-lysine), alginate, and chitosan, and encapsulating (ii) surface functionalized poly(l-lactic acid) (PLLA) microparticles were developed. We hypothesize that, while the liquified environment enhances the diffusion of essential molecules for cell survival, microparticles dispersed in the liquified core of capsules provide the physical support required for cellular functions of anchorage-dependent cells. The influence of the incorporation of PLL on the regime growth, thickness, and stability was analyzed. Results show a more resistant and thicker film with an exponential build-up growth regime. Moreover, capsules ability to support cell survival was assessed. Capsules containing microparticles revealed an enhanced biological outcome in cell metabolic activity and proliferation, suggesting their potential to boost the development of innovative biomaterial designs for bioencapsulation systems and tissue engineering products.
开发了一种液化胶囊，其特征在于（i）通过层层组装聚（L-赖氨酸）、海藻酸盐和壳聚糖的外壳，并封装（ii）表面功能化的聚（L-乳酸）（PLLA）微球。我们假设，液化环境增强了细胞存活所需的必需分子的扩散，而分散在胶囊液化核心中的微球为锚定依赖性细胞的细胞功能提供了所需的物理支撑。分析了 PLL 的掺入对膜的生长、厚度和稳定性的影响。结果表明，具有指数增长模式的膜具有更耐抗性和更厚的厚度。此外，还评估了胶囊支持细胞存活的能力。含有微球的胶囊显示出细胞代谢活性和增殖的增强的生物学结果，表明它们有可能促进用于生物封装系统和组织工程产品的创新生物材料设计的发展。
